To study the effect of transient receptor potential C6 (
TRPC6) channels on
esophageal squamous cell carcinoma (ESCC) cell lines Eca109 cell cycle and to confirm whether
TRPC6 channel is candidate radiosensitivity in vitro and in vivo.
METHODS: We chose Eca109 cell line with a strong
TRPC6 channels expression. Cell cycle was investigated after
TRPC6 channel inhibitor
SKF96365 treated with a 5 µM concentration. According to the results of cell cycle, radiation was performed.
CCK-8 test was used to test the cell proliferation. Then we performed the same study in vivo. Total of 40 male nude mice were randomly divided into four groups as follows:
SKF96365, radio, combined radio-SKF96365 and control. In
SKF96365 group, 20 mg/kg 5 µM
SKF96365 was injected into the abdominal cavity of the nude mice at day 5-11. In radiation group, the nude mice received
radiotherapy 2 Gy per day at day 7-11. In combined radio-SKF96365 group, 20 mg/kg 5 µM
SKF96365 was injected into the abdominal cavity of the nude mice at day 5-11 and 2 Gy
radiotherapy was delivered to the
tumor site at day 7-11. In control group, nude mice were injected saline into the abdominal cavity at day 5-11. General states of health were observed, the
tumor size in volume was measured with calipers two times every week. Six weeks after seeding, mice were sacrificed by neck-break. The
tumor size was measured in volume with caliper and in weigh with scale.
RESULTS: Treatment with
SKF96365 substantially increased the percentage of Eca109 cells in the G2/M phase and reduced that in G0/G1 phase in a time-dependent manner. Most of the cells (85.26%), 24 h after
SKF96365 treatment were arrested in the G2/M phase.
CCK-8 test showed that Eca109 ESCC cells received both
SKF96365 and radiation showed the worst ability of cell proliferation. The same result was obtained in vivo. Nude mice received combined radio-SKF96365 showed the smallest
tumor size and volume.
CONCLUSIONS: