[Immunoserology and RHD Genotype Analysis of DVI Type 3 Genotype Pregnant Women with Anti-D].

Abstract

OBJECTIVE:

To performe the immuneserological and RHD Genotype analyses for DVI type 3 genotype pregnemt women with anti-D.

METHODS:

RhD blood type of this pregnant women was identified by common serological methods, then the blood group specific antibodies was screened and identified; the polymerase chain reaction-sequence specific primer(PCR-SSP) was used to identify the pregnant women's RHD genotype; RhD blood group for the pregnant women, her spouse and daughter was genogrouped and genetically analyzed by multiplex ligation-dependent probe amplification(MLPA). The heredity of this family was analyzed finally.

RESULTS:

The titer of IgG anti-D in the pregnant woman serum was 1:8; the PCR-SSP showed that the 3rd to 6th exons of RHD gene were missing in the pregnant woman. the genotype of pregnant woman was identified as DVI type 3; the MLPA analysis showed that this pregnant women owned only one RHD allele with 3rd to 6th exons missed, and her genotype was identified as CDVIe/cde; her spouse was identified as CDe/CDe homozygous genotype, and her daughter as CDe/CDVIe.

CONCLUSION:

Accurate identification of RhD blood type is of great significance for a safe and effective clinical blood transfusion strategy, and for taking appropriate measures to prevent hemolytic disease of newborn (HDN) at women childbearing age.

• Authors: Qian-Ni Liang, Zhi-Jian Liao, Run-Qing Zhang, Yan-Li Ji, Guang-Ping Luo, Mei-Na Li
• Journal: Zhongguo shi yan xue ye xue za zhi (Zhongguo Shi Yan Xue Ye Xue Za Zhi) Vol. 25 Issue 6 Pg. 1810-1814 (Dec 2017) ISSN: 1009-2137 [Print] China
• PMID: 29262921 (Publication Type: Journal Article)

Chemical References

• RHO(D) antibody
• Rh-Hr Blood-Group System
• Rho(D) Immune Globulin

Topics

• Erythroblastosis, Fetal (prevention & control)
• Female
• Genotype
• Humans
• Pregnancy
• Rh-Hr Blood-Group System
• Rho(D) Immune Globulin (genetics, immunology)