Abstract |
Neointimal hyperplasia (or restenosis) is primarily attributed to excessive proliferation and migration of vascular smooth muscle cells (VSMCs). In this study, we investigated the inhibitory effects and mechanisms of ugonin J on VSMC proliferation and migration as well as neointimal formation. Cell viability and the cell-cycle distribution were, respectively, analyzed using an MTT assay and flow cytometry. Cell migration was examined using a wound-healing analysis and a transwell assay. Protein expressions and gelatinase activities were, respectively, measured using Western blot and gelatin zymography. Balloon angioplasty-induced neointimal formation was induced in a rat carotid artery model and then examined using immunohistochemical staining. Ugonin J induced cell-cycle arrest at the G0 /G1 phase and apoptosis to inhibit VSMC growth. Ugonin J also exhibited marked suppressive activity on VSMC migration. Ugonin J significantly reduced activations of focal adhesion kinase, phosphoinositide 3-kinase, v-akt murine thymoma viral oncogene homolog 1, and extracellular signal-regulated kinase 1/2 proteins. Moreover, ugonin J obviously reduced expressions and activity levels of matrix metalloproteinase-2 and matrix metalloproteinase-9. In vivo data indicated that ugonin J prevented balloon angioplasty-induced neointimal hyperplasia. Our study suggested that ugonin J has the potential for application in the prevention of balloon injury-induced neointimal formation.
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Authors | Chun-Hsu Pan, Pei-Chuan Li, Yi-Chung Chien, Wan-Ting Yeh, Chih-Chuang Liaw, Ming-Jyh Sheu, Chieh-Hsi Wu |
Journal | Phytotherapy research : PTR
(Phytother Res)
Vol. 32
Issue 2
Pg. 312-320
(Feb 2018)
ISSN: 1099-1573 [Electronic] England |
PMID | 29250830
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 John Wiley & Sons, Ltd. |
Chemical References |
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Topics |
- Angioplasty, Balloon
- Animals
- Cell Movement
(drug effects)
- Cell Proliferation
- Cells, Cultured
- Flavonoids
(pharmacology)
- Hyperplasia
(drug therapy)
- Male
- Muscle, Smooth, Vascular
(drug effects, pathology)
- Neointima
(pathology)
- Rats
- Rats, Sprague-Dawley
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