Abstract |
Glioblastoma multiforme (GBM) is the most common and deadliest of malignant brain tumors in adults. Disease development is associated with dysregulation of the cyclin D-CDK4/6-INK4-Rb pathway, resulting in increased proliferation; thus, CDK4/6 kinase inhibitors are promising candidates for GBM treatment. The recently developed CDK4/6 inhibitors, palbociclib, ribociclib, and abemaciclib, are effective in subcutaneous glioma models, but their blood-brain barrier (BBB) permeability is poor, limiting drug delivery to the central nervous system. Here, we designed and synthesized a series of novel CDK4/6 inhibitors with favorable BBB permeability for the treatment of GBM. Compound 11 exhibited a favorable pharmacological profile and significant penetration of the BBB with the Kp value of 4.10 and the Kp,uu value of 0.23 in mice after an oral dose of 10 mg/kg. IC50 values for CDK4/ cyclin D1 and CDK6/ cyclin D3 were 3 nM and 1 nM, respectively. In vivo studies with an orthotopic xenograft mouse model of GBM showed that 11 had tumor growth inhibition values ranging from 62% to 99% for doses ranging from 3.125 to 50 mg/kg, and no significant body weight loss was observed. The increase in life span based on the median survival time of vehicle-treated animals in mice administered a dose of 50 mg/kg was significant at 162% (p < 0.0001). These results suggest that compound 11 is a promising candidate for further investigation as an effective drug for the treatment of GBM.
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Authors | Lei Yin, Heng Li, Wenjian Liu, Zhenglin Yao, Zhenzhen Cheng, Huabei Zhang, Hui Zou |
Journal | European journal of medicinal chemistry
(Eur J Med Chem)
Vol. 144
Pg. 1-28
(Jan 20 2018)
ISSN: 1768-3254 [Electronic] France |
PMID | 29247857
(Publication Type: Journal Article)
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Copyright | Copyright © 2017. Published by Elsevier Masson SAS. |
Chemical References |
- Protein Kinase Inhibitors
- Cyclin-Dependent Kinase 4
- Cyclin-Dependent Kinase 6
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Topics |
- Animals
- Blood-Brain Barrier
(metabolism, pathology)
- Brain Neoplasms
(drug therapy, metabolism, pathology)
- Cell Line, Tumor
- Cyclin-Dependent Kinase 4
(antagonists & inhibitors, metabolism)
- Cyclin-Dependent Kinase 6
(antagonists & inhibitors, metabolism)
- Dogs
- Drug Design
- Glioblastoma
(drug therapy, metabolism, pathology)
- Humans
- Madin Darby Canine Kidney Cells
- Male
- Mice
- Protein Kinase Inhibitors
(chemistry, pharmacokinetics, therapeutic use)
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