Abstract | BACKGROUND: METHODS:
Suspension of AEE was prepared in 5% carboxymethyl cellulose sodium (CMC-Na). Thirty rats were divided into control, model and AEE groups. The control and model rats were fed with normal diet or HFD for 13 weeks, respectively. Rats in AEE-treated group were fed with HFD for 8 weeks to induce hyperlipidemia, and then given AEE once daily by oral gavage for 5 weeks at the dosage of 54 mg/kg body weight. After drug intervention, lipid profile analysis and oil red O staining were carried out to confirm the lipid accumulation in liver tissue. UPLC-Q-TOF/MS-based liver and feces metabonomics coupled with pathway analysis were conducted to evaluate the changes of metabolic profile and endogenous metabolites. RESULTS: In liver tissue, oral administration of AEE significantly reduced lipid droplets and the levels of triglyceride (TG) and low-density lipoprotein ( LDL). Using principal component analysis (PCA) and partial least squares-discriminate analysis (PLS-DA), distinct changes in metabolite patterns in feces and liver were observed. Liver and feces samples in control, model and AEE groups were scattered in PLS-DA score plots. 28 metabolites in liver and 22 in feces were identified as potential biomarkers related to hyperlipidemia. As possible drug targets, the perturbations of those biomarkers can be regulated by administration of AEE. CONCLUSION:
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Authors | Ning Ma, Xiwang Liu, Xiaojun Kong, Shihong Li, Zenghua Jiao, Zhe Qin, Pengcheng Dong, Yajun Yang, Jianyong Li |
Journal | Lipids in health and disease
(Lipids Health Dis)
Vol. 16
Issue 1
Pg. 240
(Dec 11 2017)
ISSN: 1476-511X [Electronic] England |
PMID | 29228968
(Publication Type: Journal Article)
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Chemical References |
- Amino Acids
- Bile Acids and Salts
- Cholesterol, LDL
- Fatty Acids
- Glycerophospholipids
- Hypolipidemic Agents
- Purines
- Triglycerides
- aspirin eugenol ester
- Eugenol
- Aspirin
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Topics |
- Administration, Oral
- Amino Acids
(metabolism)
- Animals
- Aspirin
(analogs & derivatives, pharmacology)
- Bile Acids and Salts
(metabolism)
- Body Weight
(drug effects)
- Cholesterol, LDL
(metabolism)
- Diet, High-Fat
- Eugenol
(analogs & derivatives, pharmacology)
- Fatty Acids
(metabolism)
- Feces
(chemistry)
- Glycerophospholipids
(metabolism)
- Hyperlipidemias
(drug therapy, etiology, metabolism)
- Hypolipidemic Agents
(pharmacology)
- Lipid Metabolism
(drug effects)
- Liver
(drug effects, metabolism)
- Male
- Metabolome
(drug effects)
- Principal Component Analysis
- Purines
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Triglycerides
(metabolism)
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