Stem cells rescue cardiomyopathy induced by P. gingivalis-LPS via miR-181b.

Abstract

Systemic inflammation induced by bacterial infection is one of several causative agents for cardiovascular disorders in patients with periodontal disease. Experimental results indicate that miRNAs play important roles in systemic inflammation induced by endotoxins. Further evidence states that stem cell based therapy shows potential in the treatment of inflammatory responses induced by sepsis. This study investigates if stem cells show protective effects on cardiomyocyte damage induced by porphyromonas gingivalis-LPS (Pg-LPS) through regulating miRNAs. H9c2 cardiomyoblasts and neonatal rat cardiomyocytes (NRCMs) were damaged using Pg-LPS in this study. Pg-LPS damaged H9c2 or NRCMs were then rescued using adipose-derived stem cells (ADSC). The experimental results reveal that Pg-LPS treatment is capable of inducing TLR4/NFκB axis activation, cell death signaling and IGF1R/PI3 K/Akt axis suppression. miR181b was downregulated in Pg-LPS damaged H9c2/NRCMs. All markers were improved in H9c2/NRCMs cocultured with ADSC. miR181b mimic and inhibitor confirmed that miR181b plays a central role in regulating the cardio protective effect on Pg-LPS damaged H9c2/NRCMs cocultured with ADSC. miR181b acts as potential therapeutic marker in cardiomyopathy induced by Pg-LPS. Transplantation of adipose-derived stem cells show potential in the treatment of cardiomyopathy induced by porphyromonas gingivalis endotoxin via regulation of miR181b.

• Authors: Tung-Sheng Chen, Sarnai Battsengel, Chia-Hua Kuo, Lung-Fa Pan, Yueh-Min Lin, Chun-Hsu Yao, Yueh-Sheng Chen, Feng-Huei Lin, Wei-Wen Kuo, Chih-Yang Huang
• Journal: Journal of cellular physiology (J Cell Physiol) Vol. 233 Issue 8 Pg. 5869-5876 (08 2018) ISSN: 1097-4652 [Electronic] United States
• PMID: 29226955 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © 2017 Wiley Periodicals, Inc.

Chemical References

• Cardiotonic Agents
• Lipopolysaccharides
• MicroRNAs
• NF-kappa B
• Tlr4 protein, rat
• Toll-Like Receptor 4

Topics

• Animals
• Cardiomyopathies (pathology)
• Cardiotonic Agents
• Cell Line
• Cell Survival
• Inflammation (microbiology, pathology)
• Lipopolysaccharides (toxicity)
• MicroRNAs (genetics)
• Myocytes, Cardiac (pathology)
• NF-kappa B (metabolism)
• Periodontal Diseases (microbiology, pathology)
• Porphyromonas gingivalis
• Rats
• Stem Cells (metabolism)
• Toll-Like Receptor 4 (metabolism)