Expression of SIRT1 and P53 in Rat Lens Epithelial cells in Experimentally Induced DM.

Abstract

PURPOSE:

To determine the etiopathogenesis of diabetic cataract by studying changes in relative expressions of silent information regulator protein-1 (SIRT1) and P53 in rat lens epithelial cells (LECs) in experimentally induced diabetes mellitus (DM).

METHODS:

Six-week-old male SD rats (n = 120) were randomly divided into experimental (n = 80 rats) and control (n = 40 rats) groups. DM was induced in the experimental group (diabetic model) by intraperitoneal (i.p.) injection of 60 mg/kg streptozotocin (STZ). Control group rats were injected similarly with phosphate-buffered saline (PBS). Four and eight weeks after successful induction of DM, relative expressions of SIRT1 and P53 in LECs were analyzed using quantitative real-time (qRT) fluorescence polymerase chain reaction (qRT-PCR) and Western blot analysis.

RESULTS:

Expression of both SIRT1 and P53 was observed in LECs of control and experimental group rats at 4 and 8 weeks but was significantly greater in experimental compared with control group rats (p < 0.05).

CONCLUSIONS:

Expression of both SIRT1 and P53 increases in the early stages of diabetic cataract formation, indicating that they play potentially important roles in the pathogenesis of diabetic cataract.

• Authors: Keke Xu, Shubin Wu, Zhijian Li, Hong Lou, Jiayu Yao, Hong Sun, Yin Sun, Yuxia Dai
• Journal: Current eye research (Curr Eye Res) Vol. 43 Issue 4 Pg. 493-498 (04 2018) ISSN: 1460-2202 [Electronic] England
• PMID: 29199862 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Tumor Suppressor Protein p53
• RNA
• Sirt1 protein, rat
• Sirtuin 1

Topics

• Animals
• Apoptosis
• Cataract (etiology, genetics, metabolism)
• Diabetes Mellitus, Experimental
• Epithelial Cells (metabolism, pathology)
• Gene Expression Regulation
• Lens, Crystalline (metabolism, pathology)
• Male
• RNA (genetics)
• Rats
• Rats, Sprague-Dawley
• Real-Time Polymerase Chain Reaction
• Sirtuin 1 (biosynthesis, genetics)
• Tumor Suppressor Protein p53 (biosynthesis, genetics)