Ecliptal, a promising natural lead isolated from Eclipta alba modulates adipocyte function and ameliorates metabolic syndrome.

Abstract

A swift increase has been observed in the number of individuals with metabolic syndrome worldwide. A number of natural compounds have been identified towards combating metabolic syndrome. Adding to this premise, here we report the pleiotropic activities of Ecliptal (EC); a natural compound isolated from the herb Eclipta alba. Administration of EC was shown to have prominent anti-adipogenic effects in 3T3-L1 and hMSC derived adipocytes. It was shown to activate Wnt-pathway and alter AKT signaling. Additionally, it caused cell cycle arrest and inhibited mitotic clonal expansion. EC treatment augmented mitochondrial biogenesis as well as function as estimated by expression of PGC1α, UCP-1, mitochondrial complexes and estimation of oxygen consumption rate. EC also reduced LPS-induced inflammation and tunicamycin induced ER stress. Further, EC enhanced insulin sensitivity by increasing AKT phosphorylation, inhibiting PKCα/βII phosphorylation and reducing leptin/adiponectin ratio. Finally, EC administration in Syrian golden hamsters was shown to have potent anti-dyslipidemic effects. Cumulatively, encompassing pleiotropic activities of EC, it could prove to be a potential drug candidate against obesity, insulin resistance and related metabolic syndrome.

• Authors: Abhishek Gupta, Ashok Kumar, Durgesh Kumar, Rohit Singh, Kripa Shankar, Salil Varshney, Sujith Rajan, Ankita Srivastava, Sanchita Gupta, T Narender, Anil Nilkanth Gaikwad
• Journal: Toxicology and applied pharmacology (Toxicol Appl Pharmacol) Vol. 338 Pg. 134-147 (01 01 2018) ISSN: 1096-0333 [Electronic] United States
• PMID: 29175456 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017. Published by Elsevier Inc.

Chemical References

• Plant Extracts
• Thiophenes
• ecliptal
• Proto-Oncogene Proteins c-akt

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects, physiology)
• Adipogenesis (drug effects)
• Animals
• Cell Differentiation (drug effects)
• Eclipta (chemistry)
• Endoplasmic Reticulum Stress (drug effects)
• Male
• Mesocricetus
• Metabolic Syndrome (drug therapy)
• Mice
• Mitochondria (drug effects)
• Plant Extracts (pharmacology)
• Proto-Oncogene Proteins c-akt (antagonists & inhibitors)
• Thiophenes (pharmacology)