Detection of early stage prostate cancer by using a simple carbon nanotube@paper biosensor.

Abstract

This study is an investigation for an inexpensive, simple and sensitive biosensor to detect prostate cancer using bioactivated-multi wall carbon nanotubes (MWCNTs, diameter of 20nm, length of 5µm) and a micro-pore filter paper (pore size of 0.45µm). For the immunoassay of prostate specific antigen (PSA), which is a biomarker of prostate cancer, MWCNTs were activated with PSA antibody (monoclonal antibody of the prostate specific antigen) by using N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide sodium salt (NHSS). The activated MWCNTs were deposited on the micro-pore filter paper to use as a biosensor. The prepared biosensor can assay from 0 to 500ng/mL of PSA level within 2h with the detection limit of 1.18ng/mL by the measurement of resistance change. The resistance change was caused by site selective interaction between PSA and PSA-antigen with an inexpensive bench top digital multimeter (5 1/2 digits). The detection range and sensitivity of the prepared sensor are good enough to diagnose the early stage of prostate cancer (> 4ng/mL of PSA). This paper-based biosensor is about 20 times cheaper (fabricated biosensor price: 2.4 $) and over 10 times faster than enzyme-linked immunosorbent assay (ELISA), which is a general method for the detection of a specific protein in the modernized hospitals. Furthermore, the maximum detection limit is about 50 times higher than ELISA.

• Authors: Sungkyung Ji, Myeongsoon Lee, Don Kim
• Journal: Biosensors & bioelectronics (Biosens Bioelectron) Vol. 102 Pg. 345-350 (Apr 15 2018) ISSN: 1873-4235 [Electronic] England
• PMID: 29172142 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Biomarkers, Tumor
• Nanotubes, Carbon
• Gold
• Prostate-Specific Antigen

Topics

• Biomarkers, Tumor (blood, isolation & purification)
• Biosensing Techniques
• Early Detection of Cancer
• Enzyme-Linked Immunosorbent Assay
• Gold (chemistry)
• Humans
• Male
• Metal Nanoparticles (chemistry)
• Nanotubes, Carbon (chemistry)
• Prostate-Specific Antigen (blood)
• Prostatic Neoplasms (blood)