Expression, purification and characterization of two leucine aminopeptidases of the blood fluke, Schistosoma mansoni.

Abstract

Schistosomiasis is a major neglected tropical disease (NTD) and considered the most important of the human helminthiases in terms of morbidity and mortality. Whereas treatment with praziquantel has been effective since the 1980s, the potential for the emergence of drug resistance has propelled the search for new interventions. Studies have revealed key roles of proteases in parasitic helminths during establishment of infection, tissue invasion, immune evasion, parasite feeding and development throughout the different developmental stages, pinpointing them as possible candidates. The leucine aminopeptidases (LAPs), members of the M17 family of Zn-metalloproteases, preferentially cleave leucine (Leu) residues at the N-terminal end of proteins and short peptides. These enzymes display broad proteolytic activities beyond Leu hydrolysis and are involved in processing, maturation, activation and/or degradation of substrates. As a vaccine immunogen, LAP induces protection against infection with the liver fluke Fasciola hepatica. Herein, two LAPs, SmLAP1 (Smp_030000) and SmLAP2 (Smp_083870) of the human blood fluke Schistosoma mansoni were cloned, expressed, purified and biochemically characterized. The enzymes differed in activity against diagnostic substrates, including leucine, methionine and arginine, with an optimal pH of 8.0. The activity increased in the presence of Mg+2 and Mn+2, and was inhibited by bestatin, a specific inhibitor of aminopeptidase. In addition, 1,10-phenanthroline and EDTA inhibited the enzymatic activity of SmLAP2. Finally, immunolocalization using antibodies specific for SmLAP1 and SmLAP2 identified the expression of these proteases in the egg and adult developmental stages of S. mansoni, and in intestinal epithelia, vitelline cells and sub-tegumental regions of the parasite. Characterization of schistosome proteases not only enhances understanding of the biology of schistosomes and schistosomiasis, but may also provide novel intervention approaches.

• Authors: Gabriela Maggioli, Gabriel Rinaldi, Ines Giaudrone, Patricia Berasain, José F Tort, Paul J Brindley, Carlos Carmona
• Journal: Molecular and biochemical parasitology (Mol Biochem Parasitol) Vol. 219 Pg. 17-23 (01 2018) ISSN: 1872-9428 [Electronic] Netherlands
• PMID: 29169803 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Enzyme Activators
• Enzyme Inhibitors
• Metalloproteases
• Leucyl Aminopeptidase

Topics

• Animals
• Cloning, Molecular
• Enzyme Activators (analysis)
• Enzyme Inhibitors (analysis)
• Enzyme Stability
• Fluorescent Antibody Technique
• Gene Expression
• Gene Expression Profiling
• Hydrogen-Ion Concentration
• Leucyl Aminopeptidase (biosynthesis, chemistry, genetics, isolation & purification)
• Metalloproteases (biosynthesis, genetics, isolation & purification)
• Schistosoma mansoni (enzymology)
• Substrate Specificity