Abstract |
The innate immune kinase TBK1 initiates inflammatory responses to combat infectious pathogens by driving production of type I interferons. TBK1 also controls metabolic processes and promotes oncogene-induced cell proliferation and survival. Here, we demonstrate that TBK1 activates mTOR complex 1 ( mTORC1) directly. In cultured cells, TBK1 associates with and activates mTORC1 through site-specific mTOR phosphorylation (on S2159) in response to certain growth factor receptors (i.e., EGF-receptor but not insulin receptor) and pathogen recognition receptors ( PRRs) (i.e., TLR3; TLR4), revealing a stimulus-selective role for TBK1 in mTORC1 regulation. By studying cultured macrophages and those isolated from genome edited mTOR S2159A knock-in mice, we show that mTOR S2159 phosphorylation promotes mTORC1 signaling, IRF3 nuclear translocation, and IFN-β production. These data demonstrate a direct mechanistic link between TBK1 and mTORC1 function as well as physiologic significance of the TBK1-mTORC1 axis in control of innate immune function. These data unveil TBK1 as a direct mTORC1 activator and suggest unanticipated roles for mTORC1 downstream of TBK1 in control of innate immunity, tumorigenesis, and disorders linked to chronic inflammation.
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Authors | Cagri Bodur, Dubek Kazyken, Kezhen Huang, Bilgen Ekim Ustunel, Kate A Siroky, Aaron Seth Tooley, Ian E Gonzalez, Daniel H Foley, Hugo A Acosta-Jaquez, Tammy M Barnes, Gabrielle K Steinl, Kae-Won Cho, Carey N Lumeng, Steven M Riddle, Martin G Myers Jr, Diane C Fingar |
Journal | The EMBO journal
(EMBO J)
Vol. 37
Issue 1
Pg. 19-38
(01 04 2018)
ISSN: 1460-2075 [Electronic] England |
PMID | 29150432
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | © 2017 The Authors. |
Chemical References |
- IRF3 protein, human
- Intercellular Signaling Peptides and Proteins
- Interferon Regulatory Factor-3
- MTOR protein, human
- Mechanistic Target of Rapamycin Complex 1
- Protein Serine-Threonine Kinases
- TBK1 protein, human
- TOR Serine-Threonine Kinases
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Topics |
- Animals
- Cell Nucleus
(metabolism)
- Cells, Cultured
- Cytosol
(metabolism)
- Humans
- Immunity, Innate
(drug effects)
- Intercellular Signaling Peptides and Proteins
(pharmacology)
- Interferon Regulatory Factor-3
(genetics, metabolism)
- Macrophages
(drug effects, immunology, metabolism)
- Mechanistic Target of Rapamycin Complex 1
(genetics, metabolism)
- Mice
- Phosphorylation
(drug effects)
- Protein Serine-Threonine Kinases
(genetics, metabolism)
- Protein Transport
- Signal Transduction
(drug effects)
- TOR Serine-Threonine Kinases
(genetics, metabolism)
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