Gallic Acid Reduces Blood Pressure and Attenuates Oxidative Stress and Cardiac Hypertrophy in Spontaneously Hypertensive Rats.

Abstract

Gallic acid (GA) has been reported to have beneficial effects on cancer, vascular calcification, and diabetes-induced myocardial dysfunction. We hypothesized that GA controls hypertension via oxidative stress response regulation in an animal model for essential hypertension. Spontaneously hypertensive rats (SHRs) were administered GA for 16 weeks. GA treatment lowered elevated systolic blood pressure in SHRs through the inhibition of vascular contractility and components of the renin-angiotensin II system. In addition, GA administration reduced aortic wall thickness and body weight in SHRs. In SHRs, GA attenuated left ventricular hypertrophy and reduced the expression of cardiac-specific transcription factors. NADPH oxidase 2 (Nox2) and GATA4 mRNA expression was induced in SHR hearts and angiotensin II-treated H9c2 cells; this expression was downregulated by GA treatment. Nox2 promoter activity was increased by the synergistic action of GATA4 and Nkx2-5. GA seems to regulate oxidative stress by inhibiting the DNA binding activity of GATA4 in the rat Nox2 promoter. GA reduced the GATA4-induced Nox activity in SHRs and angiotensin II-treated H9c2 cells. GA administration reduced the elevation of malondialdehyde levels in heart tissue obtained from SHRs. These findings suggest that GA is a potential therapeutic agent for treating cardiac hypertrophy and oxidative stress in SHRs.

• Authors: Li Jin, Zhe Hao Piao, Simei Sun, Bin Liu, Gwi Ran Kim, Young Mi Seok, Ming Quan Lin, Yuhee Ryu, Sin Young Choi, Hae Jin Kee, Myung Ho Jeong
• Journal: Scientific reports (Sci Rep) Vol. 7 Issue 1 Pg. 15607 (Nov 15 2017) ISSN: 2045-2322 [Electronic] England
• PMID: 29142252 (Publication Type: Journal Article)

Chemical References

• GATA4 Transcription Factor
• Gata4 protein, rat
• Homeobox Protein Nkx-2.5
• Nkx2-5 protein, rat
• Angiotensin II
• Gallic Acid
• NADPH Oxidase 2

Topics

• Angiotensin II (administration & dosage)
• Animals
• Blood Pressure (drug effects)
• Blood Pressure Determination
• Cardiomegaly (drug therapy, genetics, pathology)
• GATA4 Transcription Factor (genetics)
• Gallic Acid (administration & dosage)
• Gene Expression Regulation (drug effects)
• Heart (physiopathology)
• Homeobox Protein Nkx-2.5 (genetics)
• Humans
• Hypertension (drug therapy, genetics, physiopathology)
• Male
• NADPH Oxidase 2 (genetics)
• Oxidative Stress (drug effects)
• Rats
• Rats, Inbred SHR (genetics)