Tumor necrosis factor‑α‑mediated (TNF‑α) epithelial‑mesenchymal transition (EMT) is associated with distant
metastasis in patients with
colorectal cancer with poor prognosis. Although traditional
herbal medicines have long been used to treat
colorectal cancer, the incidence and mortality in patients with
colorectal cancer has continued to increase. Danggui‑Sayuk‑Ga‑Osuyu‑Saenggang‑Tang (
DSGOST) has long been used for treatment of
chills, while few studies have reported its anticancer effect. This study aimed to demonstrate the inhibitory effect of
DSGOST on TNF‑α‑mediated invasion and migration of
colorectal cancer HCT116 cell lines. MTT was used to measure cell viability. Wound healing and Τranswell invasion assay were used to detect migration and invasion of cells, respectively. The intracellular localization of
proteins of interest was assessed by immunocytochemistry. Western blotting was performed to determine the expression level of various
proteins. A non‑toxic dose of
DSGOST (50 µg/ml) on HCT116 cells was determined by MTT assay. Furthermore,
DSGOST prevented the TNF‑α‑induced invasive phenotype in HCT116 cells.
DSGOST inhibition of the invasive phenotype was also associated with increased expression of EMT markers. Furthermore,
DSGOST treatment blocked TNF‑α‑induced migration and invasion of HCT116 cells. In addition,
DSGOST treatment inhibited TNF‑α‑mediated nuclear translocation of Snail.
DSGOST treatment also downregulated TNF‑α‑induced phosphorylation of AKT and
glycogen synthase kinase‑3β. Therefore, the findings of the current study suggest that
DSGOST exhibits anti‑migration and anti‑invasion effects in TNF‑α‑treated HCT116 human colorectal cells.