Abstract |
FT-IR spectroscopy was used to investigate the effect of oxidative stress and to approach the mechanism on cancer bone demineralization, aortic valve mineralization and heterotopic ossification on disease development. The FT-IR spectra obtained from paediatric, adult bone and ex vivo irradiated adult healthy bone with a dose of 20Gy were compared with those of healthy bone. The increase of band intensity changes of vasCH2,vsCH2 in the region 3000-2850cm-1 depended on aging, the disease progression and the dose of irradiation. The bands at 3080cm-1 and 1744cm-1, which originate from olefinic terminal bond (v=CH) and ester carbonyl group (vROCO), respectively, indicate the influence of oxidative stress on lipid degradation and peroxidation, respectively. The new bands at about 1690cm-1 and 1516cm-1 denote the presence of β-sheet conformation of the proteins due to the diseases, confirming the increasing amount of lipophilic environment and fibril formation. Comparison of the FT-IR spectra of calcified aortic valve and hip heterotopic ossification with that of normal bones showed that in the bone-like formation the peroxide anion free radicals play an important role in the disease.
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Authors | Jane Anastassopoulou, Maria Kyriakidou, Stylianos Kyriazis, Andreas F Mavrogenis, Vasiliki Mamareli, Ioannis Mamarelis, Marianna Petra, Efthymia Malesiou, Christoforos Kotoulas, Panagiota Kolovou, Emmanouil Koutoulakis, Athina Markouizou, Theophile Theophanides |
Journal | Mechanisms of ageing and development
(Mech Ageing Dev)
Vol. 172
Pg. 107-114
(06 2018)
ISSN: 1872-6216 [Electronic] Ireland |
PMID | 29113732
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 Elsevier B.V. All rights reserved. |
Topics |
- Adult
- Aging
(metabolism, pathology)
- Aortic Valve
(metabolism, pathology)
- Aortic Valve Stenosis
(metabolism, pathology)
- Bone Neoplasms
(metabolism, pathology)
- Calcinosis
(metabolism, pathology)
- Child
- Child, Preschool
- Female
- Femur Head
(metabolism, pathology)
- Humans
- Male
- Ossification, Heterotopic
(metabolism, pathology)
- Oxidative Stress
- Spectroscopy, Fourier Transform Infrared
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