Emerging studies demonstrated that
miRNAs played fundamental roles in
lung cancer. In this study, we attempted to explore the clinical significance of the
miRNA signature in different histological subtypes of
non-small cell lung cancer (NSCLC). Three miRNome profiling datasets (GSE19945, GSE25508 and GSE51853) containing lung
squamous cell carcinoma (SCC),
lung adenocarcinoma (ADC) and large cell
lung cancer (LCLC) samples were obtained for bioinformatics and survival analysis. Moreover, pathway enrichment and coexpression network were performed to explore underlying molecular mechanism. MicroRNA-375 (miR-375), miR-203 and miR-205 were identified as differentially expressed
miRNAs (DEmiRNAs) which distinguished SCC from other NSCLC subtypes. Pathway enrichment analysis suggested that Hippo signaling pathway was combinatorically affected by above mentioned three
miRNAs. Coexpression analysis of three
miRNAs and the Hippo signaling pathway related genes were conducted based on another dataset, GSE51852. Four hub genes (TP63, RERE, TJP1 and YWHAE) were identified as the candidate targets of three
miRNAs, and three of them (TP63, TJP1 and YWHAE) were validated to be downregulated by miR-203 and miR-375, respectively. Finally, survival analysis further suggested the prognostic value of three-
miRNA signature in SCC patients. Taken together, our study compared the
miRNA profiles among three histological subtypes of NSCLC, and suggested that a three-
miRNA signature might be potential diagnostic and prognostic
biomarkers for SCC patients.