Abstract |
Nanoparticle (NP)-based combinational chemotherapy has been proposed as a potent approach for improving intracellular drug concentrations and attaining synergistic effects in colorectal cancer therapy. Here, two well-known herbal substances, Curcumin (Cur) and Chrysin (Chr), were co-encapsulated in PEGylated PLGA NPs and investigated their synergistic inhibitory effect against Caco-2 cancer cells. Characterization of nanoformulated drugs was determined using DLS, FTIR, TEM, and SEM. Drug release study was performed using dialysis method. MTT and real-time PCR assays were applied to evaluate the cytotoxic effects of free and nano-encapsulated drugs on expression level of hTERT in Caco-2 cells. The results showed that free drugs and nano-formulations exhibited a dose-dependent cytotoxicity against Caco-2 cells and especially, Cur-Chr-PLGA/PEG NPs had more synergistic antiproliferative effect and significantly arrested the growth of cancer cells than the other groups (P < 0.05). Real-time PCR results revealed that Cur, Chr, and combination of Cur-Chr in free and encapsulated forms inhibited hTERT gene expression. Also, it was found that Cur-Chr-PLGA/PEG NPs than free combination forms could further decline hTERT expression in all concentration (P < 0.05). In summary, our study represents the first report of nano-combinational application of the natural herbal substances with a one-step fabricated codelivery system for effective colorectal cancer combinational chemotherapy.
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Authors | Javid Lotfi-Attari, Younes Pilehvar-Soltanahmadi, Mehdi Dadashpour, Shahriar Alipour, Raana Farajzadeh, Shahrzad Javidfar, Nosratollah Zarghami |
Journal | Nutrition and cancer
(Nutr Cancer)
2017 Nov-Dec
Vol. 69
Issue 8
Pg. 1290-1299
ISSN: 1532-7914 [Electronic] United States |
PMID | 29083232
(Publication Type: Journal Article)
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Chemical References |
- Flavonoids
- Polyesters
- polyethylene glycol-poly(lactide-co-glycolide)
- chrysin
- Polyethylene Glycols
- TERT protein, human
- Telomerase
- Curcumin
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Topics |
- Caco-2 Cells
- Colorectal Neoplasms
(drug therapy)
- Curcumin
(pharmacology)
- Drug Delivery Systems
- Drug Liberation
- Drug Synergism
- Flavonoids
(pharmacology)
- Humans
- Nanoparticles
(chemistry)
- Particle Size
- Polyesters
(chemistry)
- Polyethylene Glycols
(chemistry)
- Telomerase
(genetics, metabolism)
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