Transdifferentiation of human MNNG/HOS osteosarcoma cells into vascular endothelial cells in vitro and in vivo.

Abstract

The transdifferentiation of cancer cells into other types of cells in several types of tissues or organs has been studied. However, whether human osteosarcoma MNNG/HOS cells can transdifferentiate into other types of cells has seldom been reported. Meanwhile, the mechanism of tumor angiogenesis is still disputed, and whether MNNG/HOS cells participate in angiogenesis in osteosarcoma remains unknown. In the present study, the investigation was divided into two parts: in vitro and in vivo. In vitro, we cultivated MNNG/HOS cells under hypoxic conditions for 4 days and found that they typically showed a characteristic 'flagstone' appearance as cultured vascular endothelial cells (VECs). MNNG/HOS cells that were cultivated on Matrigel under hypoxic conditions gradually formed tubular-like structures. Furthermore, when cultured under hypoxic conditions for 4 days, MNNG/HOS cells also transcribed and expressed several molecular markers of VECs (CD31, CD34 and vWF). In vivo, MNNG/HOS cells (1x106 cells) were cultivated under hypoxic conditions and subcutaneously injected into nude mice; the mice were sacrificed 49 days after inoculation. Immunohistochemical staining with anti-human CD31 antibody showed evidence of tumor angiogenesis in human osteosarcoma MNNG/HOS cells. The results demonstrated that MNNG/HOS cells can transdifferentiate into vascular endothelial cell-like cells in vitro and in vivo.

• Authors: Xinwen Wang, Weifeng Xu, Shenglin Wang, Feqiang Yu, Jinyi Feng, Xinwu Wang, Lurong Zhang, Jianhua Lin
• Journal: Oncology reports (Oncol Rep) Vol. 38 Issue 5 Pg. 3153-3159 (Nov 2017) ISSN: 1791-2431 [Electronic] Greece
• PMID: 29048647 (Publication Type: Journal Article)

Chemical References

• Antigens, CD34
• Drug Combinations
• Laminin
• Platelet Endothelial Cell Adhesion Molecule-1
• Proteoglycans
• Von Willebrand antigen
• von Willebrand Factor
• matrigel
• Collagen

Topics

• Animals
• Antigens, CD34 (genetics)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Cell Transdifferentiation (genetics)
• Collagen (chemistry)
• Drug Combinations
• Endothelial Cells (metabolism, pathology)
• Gene Expression Regulation, Neoplastic (genetics)
• Humans
• Laminin (chemistry)
• Mice
• Neovascularization, Pathologic (genetics, pathology)
• Osteosarcoma (genetics, pathology)
• Platelet Endothelial Cell Adhesion Molecule-1 (genetics)
• Proteoglycans (chemistry)
• Xenograft Model Antitumor Assays
• von Willebrand Factor (genetics)