Abstract |
The use of gold nanorods (AuNRs) that produce heat in response to near infrared (NIR) irradiation is an attractive approach to cancer photothermal therapy. AuNRs are usually prepared by using a highly toxic detergent: cetyltrimethylammonium bromide ( CTAB). Thus, the removal of CTAB from the reaction mixture, and further stabilization of the surface of the AuNRs is required. In the present study, AuNRs were encapsulated in a multifunctional envelope-type nano device (AuNR-MEND) formed with an SS-cleavable and pH-activated lipid-like material. In the process of encapsulation, AuNRs were first stabilized with bovine serum albumin (AuNR-BSA), and then further encapsulated in the lipid envelope by the ethanol dilution method. The in vitro photothermal cytotoxicity of AuNR-MEND was further demonstrated on 4T1 breast cancer cells. After NIR radiation, the temperature of the medium was increased to approximately 60°C, and cell viability was drastically decreased to approximately 11%. However, this cytotoxic effect cannot simply be explained by medium heating. It therefore appears that intracellular delivery of the AuNRs is a key factor for achieving a high degree of cytotoxicity. Dose dependent cytotoxicity data revealed that a higher dose of AuNR-MEND resulted in the complete destruction of the cells when they were subjected to NIR irradiation, while the cell survival rate reached a plateau at 30% in the case of AuNR-BSA. Apoptosis was induced after treatment with the nanoparticles. AuNR-MEND showed superior cellular uptake activity over AuNR-BSA. Thus, delivering AuNR by means of functionalized lipid nanoparticles represents a promising approach to induce NIR-triggered apoptosis.
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Authors | West Kristian D Paraiso, Hiroki Tanaka, Yusuke Sato, Daiki Shirane, Noriyuki Suzuki, Yasumitsu Ogra, Kota Tange, Yuta Nakai, Hiroki Yoshioka, Hideyoshi Harashima, Hidetaka Akita |
Journal | Colloids and surfaces. B, Biointerfaces
(Colloids Surf B Biointerfaces)
Vol. 160
Pg. 715-723
(Dec 01 2017)
ISSN: 1873-4367 [Electronic] Netherlands |
PMID | 29035819
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 Elsevier B.V. All rights reserved. |
Chemical References |
- Lipids
- Serum Albumin, Bovine
- Gold
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Topics |
- Animals
- Cattle
- Cell Line, Tumor
- Cell Survival
(drug effects, radiation effects)
- Drug Compounding
- Gold
(chemistry, pharmacology)
- Lipids
(chemistry)
- Metal Nanoparticles
(chemistry, ultrastructure)
- Mice
- Microscopy, Electron, Transmission
- Nanotubes
(chemistry, ultrastructure)
- Phototherapy
(methods)
- Serum Albumin, Bovine
(chemistry)
- Temperature
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