Genipin inhibits allergic responses in ovalbumin-induced asthmatic mice.

Abstract

Genipin is a natural compound isolated from the fruit of Gardenia jasminoides with various pharmacological effects. In this study, we investigated whether genipin effectively alleviates allergic responses in a murine model of ovalbumin (OVA)-induced asthma. The mice were administered an intraperitoneal injection of OVA on day 0 and 14 to boost the immune response; genipin was then administered from day 18 to 23 by oral gavage. On days 21 to 23, mice were OVA-challenged using am ultrasonic nebulizer, and airway hyperresponsiveness (AHR) was determined on day 24 by plethysmography. Genipin significantly reduced the inflammatory cell count in bronchoalveolar lavage fluids (BALF) and AHR, which were accompanied by lower interleukin-5 (IL-5), IL-13 and OVA-specific immunoglobulin (Ig) E levels in the BALF or serum from OVA-induced asthmatic mice. In histology, genipin significantly decreased airway inflammation and mucus hypersecretion in OVA-induced asthmatic mice. Additionally, genipin inhibited OVA-induced increases in the expression of inducible nitric oxide synthase and cyclooxygenase-2 proteins. Further, genipin reduced the activity and protein levels of matrix metalloproteinase-9 in lung tissue from OVA induced asthmatic mice. Overall, genipin effectively alleviated the asthmatic inflammatory response in an OVA-induced asthmatic model. Therefore, our results suggest that genipin has therapeutic potential for treating asthma.

• Authors: Je-Won Ko, Na-Rae Shin, Sung-Hyeuk Park, Young-Kwon Cho, Jong-Choon Kim, Chang-Seob Seo, In-Sik Shin
• Journal: International immunopharmacology (Int Immunopharmacol) Vol. 53 Pg. 49-55 (Dec 2017) ISSN: 1878-1705 [Electronic] Netherlands
• PMID: 29035815 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• IL5 protein, human
• Interleukin-13
• Interleukin-5
• Iridoids
• Immunoglobulin E
• Ovalbumin
• genipin
• Nitric Oxide Synthase Type II
• Matrix Metalloproteinase 9

Topics

• Animals
• Anti-Inflammatory Agents (therapeutic use)
• Asthma (drug therapy)
• Bronchial Hyperreactivity (drug therapy)
• Bronchoalveolar Lavage Fluid (immunology)
• Disease Models, Animal
• Female
• Gardenia (immunology)
• Hypersensitivity (drug therapy)
• Immunoglobulin E (blood)
• Inflammation (drug therapy)
• Interleukin-13 (metabolism)
• Interleukin-5 (metabolism)
• Iridoids (therapeutic use)
• Lung (drug effects, pathology)
• Matrix Metalloproteinase 9 (metabolism)
• Mice
• Mice, Inbred BALB C
• Nitric Oxide Synthase Type II (metabolism)
• Ovalbumin (immunology)