In 2014, non-invasive prenatal testing (
NIPT) for
trisomies 21, 18 and 13 was added to the Dutch prenatal screening program as part of the TRIDENT study. Most (85%) pregnant Dutch women are counselled for prenatal
aneuploidy screening by primary care midwives. This will remain when
NIPT is implemented as a first-tier screening test. We therefore investigated midwife counsellors': 1) Knowledge about
NIPT; 2) Attitudes towards
NIPT as first-tier screening test; and 3) Experiences with informing clients about
NIPT. Between April-June 2015, an online questionnaire to assess knowledge about
NIPT, attitudes towards
NIPT, and experiences with
NIPT was completed by 436 Dutch primary care midwives. We found that 59% midwives answered ≥7 of 8 knowledge questions correctly. Continuing professional education attendance and more positive attitudes towards prenatal screening for
Down syndrome were positively associated with the total knowledge score (β = 0.261; p = 0.007 and β = 0.204; p = 0.015, respectively). The majority (67%) were in favor of replacing First trimester Combined Test with
NIPT, although 41% preferred to maintain a nuchal translucency measurement alongside
NIPT. We conclude that midwives demonstrated solid knowledge about
NIPT that may still be improved in some areas. Dutch midwives overwhelmingly support the integration of
NIPT as a first-tier screening test.