[Expression of pituitary tumor-transforming gene 1 during the development of androgen-independent prostate cancer].

Abstract

OBJECTIVE:

To explore the expression of pituitary tumor transforming gene 1 (PTTG1) during the transformation of prostate cancer from androgen-dependent (ADPC) to androgen-independent (AIPC).

METHODS:

We established an AIPC cell model LNCaP-AI by culturing the androgen-dependent LNCaP cell line in the hormone-deprived medium for over 3 months. The cell model was verified and the PTTG1 expression in the LNCaP cells was detected by Western blot and RT-PCR during hormone deprivation.

RESULTS:

The AIPC cell model LNCaP-AI was successfully established. The PTTG1 expression was gradually increased in the LNCaP cells with the prolonged time of hormone deprivation and the expressions of matrix metalloproteinases MMP-2 and -9 were elevated at the same time.

CONCLUSIONS:

The expression of PTTG1 is increased gradually in AIPC, which may be a target of gene therapy for advanced prostate cancer.

• Authors: Xi-Liang Cao, Xiao-Ming Song, Wen-Chao Yu, Yong-Qiang Chen, Yang-Yang Wei, Yong-Liang Liu, Ke-Quan Lu
• Journal: Zhonghua nan ke xue = National journal of andrology (Zhonghua Nan Ke Xue) Vol. 22 Issue 8 Pg. 686-691 (Aug 2016) ISSN: 1009-3591 [Print] China
• PMID: 29019223 (Publication Type: Journal Article)

Chemical References

• Securin
• pituitary tumor-transforming protein 1, human
• Matrix Metalloproteinase 2
• Matrix Metalloproteinase 9

Topics

• Blotting, Western
• Cell Line, Tumor
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• Neoplasms, Hormone-Dependent
• Prostatic Neoplasms (enzymology, genetics)
• Securin (genetics)