Antigen-specificity of antiretinal antibodies in patients with noninfectious uveitis.

Abstract

OBJECTIVE:

Antiretinal antibodies (ARAs) have previously been described in noninfectious uveitis. However, the antigen specificity of these ARAs has not been investigated. The purpose of this study was to identify antigen-specific ARAs in noninfectious uveitis.

METHODS:

A total of 18 patients with noninfectious uveitis were enrolled. Surface plasmon resonance was used to measure binding responses of patient and control sera against several uveitogenic proteins: recoverin, S-antigen, interphotoreceptor retinoid binding (IRBP), retinal-pigment-epithelium-specific 65-kDa protein (RPE65), tyrosinase-related protein 1 (TRYP1), and tyrosinase-related protein 2 (TRYP2).

RESULTS:

The frequency of ARA positivity against S-antigen, IRBP, RPE65, TYRP1, and TYRP2 in patients with uveitis did not differ significantly from that of normal controls. However, ARA positivity for recoverin was more frequently observed in patients with uveitis (p = 0.002). A total of 10 patients in the uveitis cohort had birdshot chorioretinopathy, and all 10 were positive for anti-recoverin ARAs.

CONCLUSIONS:

Patients with noninfectious uveitis have increased frequency of ARA positivity against recoverin. This ARA deserves further investigations as a potential biomarker and pathogenic agent in noninfectious uveitis, especially in birdshot chorioretinopathy.

• Authors: Ebrima Gibbs, Joanne Matsubara, Sijia Cao, Jing Cui, Farzin Forooghian
• Journal: Canadian journal of ophthalmology. Journal canadien d'ophtalmologie (Can J Ophthalmol) Vol. 52 Issue 5 Pg. 463-467 (Oct 2017) ISSN: 1715-3360 [Electronic] England
• PMID: 28985805 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 Canadian Ophthalmological Society. Published by Elsevier Inc. All rights reserved.

Chemical References

• Arrestin
• Autoantibodies
• Autoantigens
• Epitopes
• Eye Proteins
• RCVRN protein, human
• Retinol-Binding Proteins
• interstitial retinol-binding protein
• Recoverin
• retinoid isomerohydrolase
• GZMK protein, human
• Granzymes
• PRSS1 protein, human
• Trypsin
• cis-trans-Isomerases

Topics

• Adult
• Aged
• Arrestin (immunology)
• Autoantibodies (blood)
• Autoantigens (immunology)
• Epitopes (immunology)
• Eye Proteins (immunology)
• Female
• Granzymes (immunology)
• Humans
• Male
• Middle Aged
• Prospective Studies
• Recoverin (immunology)
• Retina (immunology)
• Retinol-Binding Proteins (immunology)
• Surface Plasmon Resonance
• Trypsin (immunology)
• Uveitis (immunology)
• cis-trans-Isomerases (immunology)