Inhibition of autophagy modulates astaxanthin and total fatty acid biosynthesis in Chlorella zofingiensis under nitrogen starvation.

Abstract

The present study showed that inhibition of autophagy significantly increased cellular levels of reactive oxygen species in Chlorella zofingiensis under nitrogen starvation. This was accompanied with increased expression of PSY, and enhanced accumulation of astaxanthin after 48h of cultivation. Nevertheless, the proportion of astaxanthin in secondary carotenoids remained unchanged. Meanwhile, the expression level of ACCase was also elevated in the 3-MA-treated cells compared to the control despite a >20% lower content of fatty acid in the former than the latter. This phenomenon might be due to inhibition of recycling of cellular components by 3-MA and suggests the potential involvement of post-transcriptional regulation in fatty acid biosynthesis. In summary, our work has been the first to report a potentially important role of autophagy in fatty acid and astaxanthin accumulation in C. zofingiensis under stress conditions. The findings might provide valuable insights to guide further research in this area.

• Authors: Zhao Zhang, Dongzhe Sun, Ka-Wing Cheng, Feng Chen
• Journal: Bioresource technology (Bioresour Technol) Vol. 247 Pg. 610-615 (Jan 2018) ISSN: 1873-2976 [Electronic] England
• PMID: 28985609 (Publication Type: Journal Article)
• Copyright: Copyright © 2017. Published by Elsevier Ltd.

Chemical References

• Fatty Acids
• Xanthophylls
• astaxanthine
• Nitrogen

Topics

• Autophagy
• Chlorella
• Fatty Acids
• Nitrogen
• Xanthophylls