Abstract |
Cigarette smoke causes insulin resistance, which is associated with type 2 diabetes mellitus (T2DM). However, the mechanism by which this occurs remains poorly understood. Because the involvement of microRNAs ( miRNAs) in the development of insulin resistance is largely unknown, we investigated, in hepatocytes, the roles of miR-191 in cigarette smoke extract (CSE)-induced insulin resistance. In L-02 cells, CSE not only decreased glucose uptake and glycogen levels but also reduced levels of insulin receptor substrate-1 (IRS-1) and Akt activation, effects that were blocked by SC79, an activator of Akt. CSE also increased miR-191 levels in L-02 cells. Furthermore, the inhibition of miR-191 blocked the decreases of IRS-1 and p-Akt levels, which antagonized the decreases of glucose uptake and glycogen levels in L-02 cells induced by CSE. These results reveal a mechanism by which miR-191 is involved in CSE-induced hepatic insulin resistance via the IRS-1/Akt signaling pathway, which helps to elucidate the mechanism for cigarette smoke-induced T2DM.
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Authors | Qianlei Yang, Yan Cui, Fei Luo, Xinlu Liu, Qiushi Wang, Jun Bai, Faqin Dong, Qian Sun, Lu Lu, Hui Xu, Junchao Xue, Chao Chen, Quanyong Xiang, Qizhan Liu, Qingbi Zhang |
Journal | Environmental science and pollution research international
(Environ Sci Pollut Res Int)
Vol. 25
Issue 23
Pg. 22400-22407
(Aug 2018)
ISSN: 1614-7499 [Electronic] Germany |
PMID | 28963693
(Publication Type: Journal Article)
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Chemical References |
- IRS1 protein, human
- Insulin Receptor Substrate Proteins
- MIRN191 microRNA, human
- MicroRNAs
- Glycogen
- Proto-Oncogene Proteins c-akt
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Topics |
- Cell Line
- Cigarette Smoking
(adverse effects)
- Diabetes Mellitus, Type 2
(etiology, metabolism)
- Glycogen
(metabolism)
- Hepatocytes
(drug effects, metabolism)
- Humans
- Insulin Receptor Substrate Proteins
(metabolism)
- Insulin Resistance
(genetics)
- MicroRNAs
(genetics, metabolism)
- Proto-Oncogene Proteins c-akt
(metabolism)
- Signal Transduction
(drug effects)
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