[Intervention effects of Jiaotai pills on PCPA-induced insomnia in rats].

Abstract

To elucidate the intervention effects of Jiaotai pills(JTP) on p-chlorophenylalanine (PCPA)-induced insomnia in rats and its underlying mechanism, the insomnia model was established by single intraperitoneal injection with PCPA in rats. The locomotor activity of rats was observed, and the levels of nerve growth factor(NGF) in hypothalamus, hippocampus, prefrontal cortex and serum of rats were determined by using ELISA. Moreover, a proton nuclear magnetic resonance(¹H-NMR)-based metabonomic approach was developed to profile insomnia-related metabolites in rat serum and hippocampus and analyze the intervention effects of JTP on changes in underlying biomarkers related to locomotor activity, NGF and insomnia. According to the results, JTP could significantly suppress the locomotor activity of insomnia rats, and increase the NGF levels in hypothalamus, hippocampus, prefrontal cortex and serum of rats with insomnia. The disturbed metabolic state associated with PCPA-induced insomnia in rat serum and hippocampus could be intervened by JTP. Meanwhile, six and five potential biomarkers related to insomnia in rat serum and hippocampus were reversed by administration of JTP. In conclusion, the current study demonstrated that JTP had protective effects against PCPA-induced insomnia in rats, which was probably correlated with regulation of NGF level and metabolism of amino acids, lipids and choline.

• Authors: He Yue, Xiang-Yu Zhou, Chun-Yuan Li, Zhong-Jie Zou, Shu-Mei Wang, Sheng-Wang Liang, Meng-Juan Gong
• Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi) Vol. 41 Issue 18 Pg. 3451-3456 (Sep 2016) ISSN: 1001-5302 [Print] China
• PMID: 28925131 (Publication Type: Journal Article)
• Copyright: Copyright© by the Chinese Pharmaceutical Association.

Chemical References

• Drugs, Chinese Herbal
• jiaotai
• Nerve Growth Factor

Topics

• Animals
• Drugs, Chinese Herbal (pharmacology)
• Hippocampus (drug effects)
• Nerve Growth Factor (metabolism)
• Rats
• Sleep Initiation and Maintenance Disorders (chemically induced, drug therapy)