Anti-Obesity Activity of Saringosterol Isolated from Sargassum muticum (Yendo) Fensholt Extract in 3T3-L1 Cells.

Abstract

Saringosterol, a steroid isolated from Sargassum muticum, a brown edible alga widely distributed on the seashores of southern and eastern Korea, has been shown to exhibit anti-obesity effect. In this study, we investigated the anti-obesity activity of saringosterol through various experiments. The inhibitory effect of saringosterol on adipogenesis was evaluated via Oil Red O staining in 3T3-L1 preadipocytes. After confirming that saringosterol is not cytotoxic to these cells by using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, the effect of saringosterol on the expression of various adipogenesis-related genes was analyzed via quantitative real-time polymerase chain reaction and western blotting. We demonstrated that saringosterol dose dependently inhibited adipocyte differentiation and expression of adipogenic marker genes such as adipocyte fatty acid-binding protein, adiponectin, resistin, and fatty acid synthase in 3T3-L1 cells. In addition, saringosterol significantly inhibited the mRNA and protein expression of peroxisome proliferator-activated receptor γ and CCAAT enhancer-binding protein α in 3T3-L1 cells. Collectively, these findings indicate that saringosterol isolated from S. muticum exhibits anti-obesity effect by inhibiting the expression of adipogenic transcription factors and marker genes and that it may be developed as a drug to suppress adipogenesis. Copyright © 2017 John Wiley & Sons, Ltd.

• Authors: Jung A Lee, Young-Rak Cho, Seong Su Hong, Eun-Kyung Ahn
• Journal: Phytotherapy research : PTR (Phytother Res) Vol. 31 Issue 11 Pg. 1694-1701 (Nov 2017) ISSN: 1099-1573 [Electronic] England
• PMID: 28921681 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 John Wiley & Sons, Ltd.

Chemical References

• Adiponectin
• Anti-Obesity Agents
• CCAAT-Enhancer-Binding Protein-alpha
• Fatty Acid-Binding Proteins
• PPAR gamma
• Plant Extracts
• Resistin
• Transcription Factors
• saringosterol
• Stigmasterol
• Fatty Acid Synthases

Topics

• 3T3-L1 Cells
• Adipocytes (drug effects)
• Adipogenesis (drug effects)
• Adiponectin (metabolism)
• Animals
• Anti-Obesity Agents (pharmacology)
• CCAAT-Enhancer-Binding Protein-alpha (metabolism)
• Cell Differentiation (drug effects)
• Fatty Acid Synthases (metabolism)
• Fatty Acid-Binding Proteins (metabolism)
• Mice
• PPAR gamma (metabolism)
• Plant Extracts (pharmacology)
• Real-Time Polymerase Chain Reaction
• Republic of Korea
• Resistin (metabolism)
• Sargassum (chemistry)
• Stigmasterol (analogs & derivatives, pharmacology)
• Transcription Factors (metabolism)