Versican is produced by Trif- and type I interferon-dependent signaling in macrophages and contributes to fine control of innate immunity in lungs.

Abstract

Growing evidence suggests that versican is important in the innate immune response to lung infection. Our goal was to understand the regulation of macrophage-derived versican and the role it plays in innate immunity. We first defined the signaling events that regulate versican expression, using bone marrow-derived macrophages (BMDMs) from mice lacking specific Toll-like receptors (TLRs), TLR adaptor molecules, or the type I interferon receptor (IFNAR1). We show that LPS and polyinosinic-polycytidylic acid [poly(I:C)] trigger a signaling cascade involving TLR3 or TLR4, the Trif adaptor, type I interferons, and IFNAR1, leading to increased expression of versican by macrophages and implicating versican as an interferon-stimulated gene. The signaling events regulating versican are distinct from those for hyaluronan synthase 1 (HAS1) and syndecan-4 in macrophages. HAS1 expression requires TLR2 and MyD88. Syndecan-4 requires TLR2, TLR3, or TLR4 and both MyD88 and Trif. Neither HAS1 nor syndecan-4 is dependent on type I interferons. The importance of macrophage-derived versican in lungs was determined with LysM/Vcan-/- mice. These studies show increased recovery of inflammatory cells in the bronchoalveolar lavage fluid of poly(I:C)-treated LysM/Vcan-/- mice compared with control mice. IFN-β and IL-10, two important anti-inflammatory molecules, are significantly decreased in both poly(I:C)-treated BMDMs from LysM/Vcan-/- mice and bronchoalveolar lavage fluid from poly(I:C)-treated LysM/Vcan-/- mice compared with control mice. In short, type I interferon signaling regulates versican expression, and versican is necessary for type I interferon production. These findings suggest that macrophage-derived versican is an immunomodulatory molecule with anti-inflammatory properties in acute pulmonary inflammation.

• Authors: Mary Y Chang, Inkyung Kang, Michael Gale Jr, Anne M Manicone, Michael G Kinsella, Kathleen R Braun, Tara Wigmosta, William C Parks, William A Altemeier, Thomas N Wight, Charles W Frevert
• Journal: American journal of physiology. Lung cellular and molecular physiology (Am J Physiol Lung Cell Mol Physiol) Vol. 313 Issue 6 Pg. L1069-L1086 (Dec 01 2017) ISSN: 1522-1504 [Electronic] United States
• PMID: 28912382 (Publication Type: Journal Article)
• Copyright: Copyright © 2017 the American Physiological Society.

Chemical References

• Adaptor Proteins, Vesicular Transport
• IL10 protein, mouse
• Ifnar1 protein, mouse
• Lipopolysaccharides
• Sdc4 protein, mouse
• Syndecan-4
• TICAM-1 protein, mouse
• Toll-Like Receptors
• Vcan protein, mouse
• lipopolysaccharide, Escherichia coli O111 B4
• Versicans
• Interleukin-10
• Receptor, Interferon alpha-beta
• Interferon-beta
• Hyaluronan Synthases

Topics

• Adaptor Proteins, Vesicular Transport (genetics, immunology)
• Animals
• Hyaluronan Synthases (genetics, immunology)
• Immunity, Innate
• Interferon-beta (immunology)
• Interleukin-10 (genetics, immunology)
• Lipopolysaccharides (toxicity)
• Lung (immunology)
• Macrophages, Alveolar (immunology)
• Mice
• Mice, Knockout
• Receptor, Interferon alpha-beta (genetics, immunology)
• Syndecan-4 (genetics, immunology)
• Toll-Like Receptors (genetics, immunology)
• Versicans (genetics, immunology)