The Effects of Barrier Agents in Postoperative Pelvic Adhesion Formation: A Comparative Study of a Temperature-Sensitive Poloxamer-Based Solution/Gel and a Hyaluronic Acid-Based Solution in a Rat Uterine Horn Model.

Abstract

OBJECTIVE:

The aim is to evaluate the efficacy of poloxamer/alginate/CaCl2 mixture (PACM) solution/gel and hyaluronic acid/carboxymethylcellulose (HA-CMC) solution for reducing pelvic adhesion in a rat uterine horn model.

STUDY DESIGN:

A total of 48 females, Sprague Dawley rats, were divided into three groups according to the applied materials. A uterine horn adhesion model was created. The control group (group CO; n = 16) received no special materials except saline infusion. The experimental groups were treated with 1.0 mL HA-CMC solution (group HA-CMC; n = 16) or 1.0 mL PACM solution/gel (group PACM; n = 16). Adhesion scores were evaluated with macroscopic, microscopic, and immunohistochemistry grading 14 days postoperatively.

RESULTS:

Macroscopic adhesion scores, including extent, severity, and total scores in group HA-CMC and group PACM, were significantly lower than those in group CO (P < .001). Among these three categories of scoring, group PACM had a significantly lower score than did group HA-CMC in adhesion severity (P = .025). In the microscopic adhesion score analysis, the fibrosis scores in group HA-CMC and group PACM were also significantly lower than that of group CO. In group PACM, the fibrosis score was significantly lower than that of group HA-CMC. There were no statistical differences across all three groups in the microscopic inflammation and immunohistochemistry staining.

CONCLUSION:

Both HA-CMC solution and PACM solution/gel effectively reduced adhesion formation. PACM solution/gel was superior to HA-CMC solution in preventing pelvic adhesion, especially in adhesion severity and microscopic fibrosis.

• Authors: Hyeon-Ji Kim, Hyun Kang, Mi-Kyung Kim, Seung-Su Han
• Journal: Journal of laparoendoscopic & advanced surgical techniques. Part A (J Laparoendosc Adv Surg Tech A) Vol. 28 Issue 2 Pg. 134-139 (Feb 2018) ISSN: 1557-9034 [Electronic] United States
• PMID: 28910183 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Alginates
• Hexuronic Acids
• Poloxamer
• Glucuronic Acid
• Hyaluronic Acid
• Carboxymethylcellulose Sodium

Topics

• Alginates (pharmacology)
• Animals
• Carboxymethylcellulose Sodium (pharmacology)
• Female
• Glucuronic Acid (pharmacology)
• Hexuronic Acids (pharmacology)
• Hyaluronic Acid (pharmacology)
• Immunohistochemistry
• Pelvis (pathology)
• Poloxamer (pharmacology)
• Postoperative Complications (prevention & control)
• Rats
• Rats, Sprague-Dawley
• Temperature
• Tissue Adhesions (prevention & control)
• Uterine Diseases (prevention & control)
• Uterus (drug effects, pathology)