Mycothiol acetyltransferase (Rv0819) of Mycobacterium tuberculosis is a potential biomarker for direct diagnosis of tuberculosis using patient serum specimens.

Abstract

Mycobacterium tuberculosis infection constitutes a global threat that results in significant morbidity and mortality worldwide. Efficient and early diagnosis of tuberculosis (TB) is of paramount importance for successful treatment. The aim of the current study is to investigate the mycobacterial mycothiol acetyltransferase Rv0819 as a potential novel biomarker for the diagnosis of active TB infection. The gene encoding Rv0819 was cloned and successfully expressed in Escherichia coli. The recombinant Rv0819 was purified using metal affinity chromatography and was used to raise murine polyclonal antibodies against Rv0819. The raised antibodies were employed for direct detection of Rv0819 in patient serum samples using dot blot assay and competitive enzyme-linked immunosorbent assay (ELISA). Serum samples were obtained from 68 confirmed new TB patients and 35 healthy volunteers as negative controls. The dot blot assay showed sensitivity of 64·7% and specificity of 100%, whereas the competitive ELISA assay showed lower sensitivity (54·4%) and specificity (88·57%). The overall sensitivity of the combined results of the two tests was found to be 89·7%. Overall, the mycobacterial Rv0819 is a potential TB serum biomarker that can be exploited, in combination with other TB biomarkers, for efficient and reliable diagnosis of active TB infection.

SIGNIFICANCE AND IMPACT OF THE STUDY:

The early and accurate diagnosis of tuberculosis infection is of paramount importance for initiating treatment and avoiding clinical complications. Most current diagnostic tests have poor sensitivity and/or specificity and in many cases they are too expensive for routine diagnostic testing in resource-limited settings. In the current study, we examined a novel mycobacterial serum biomarker, namely mycothiol acetyltransferase Rv0819. The antigen was detectable in serum specimens of a significant number of tuberculosis patients. This article proves the importance of Rv0819 and paves the way towards its future use as a useful diagnostic marker for tuberculosis infection.

• Authors: H Zeitoun, M Bahey-El-Din, M A Kassem, H M Aboushleib
• Journal: Letters in applied microbiology (Lett Appl Microbiol) Vol. 65 Issue 6 Pg. 504-511 (Dec 2017) ISSN: 1472-765X [Electronic] England
• PMID: 28905401 (Publication Type: Journal Article)
• Copyright: © 2017 The Society for Applied Microbiology.

Chemical References

• Antigens, Bacterial
• Biomarkers
• Glycopeptides
• mycothiol
• Inositol
• Acetyltransferases
• acetyl-CoA 1-D-myo-inosityl 2-L-cysteinylamido-2-deoxy-alpha-D-glucopyranoside acetyltransferase
• Cysteine

Topics

• Acetyltransferases (blood, genetics)
• Adult
• Aged
• Antigens, Bacterial (immunology)
• Biomarkers (blood)
• Cloning, Molecular
• Cysteine (metabolism)
• Early Diagnosis
• Enzyme-Linked Immunosorbent Assay (methods)
• Escherichia coli (genetics, metabolism)
• Female
• Glycopeptides (metabolism)
• Humans
• Inositol (metabolism)
• Male
• Middle Aged
• Mycobacterium tuberculosis (enzymology, genetics)
• Sensitivity and Specificity
• Tuberculosis, Pulmonary (diagnosis)
• Young Adult