Abstract | BACKGROUND: MATERIALS AND METHODS: In this study, we used quantitative real-time PCR (qRT-PCR) to measure BACH1 expression in prostate adenocarcinoma tissues and two metastasis-derived prostate cancer cell lines, DU145 and LNCaP. We also used immunohistochemical (IHC) staining to measure BACH1 protein expression in prostate adenocarcinoma and matched normal tissue samples. In the following BACH1 expression was silenced in DU145 cells using siRNA as well. Knockdown was confirmed by qRT-PCR and Western blotting. The cytotoxic effects of BACH1-siRNA on DU145 cells were determined using an MTT assay. The migration and invasive capacity of DU145 cells were examined by scratch wound healing assay and matrigel invasion assay, respectively. We also used qRT-PCR to study the effect of BACH1 silencing on the expression levels of metastasis-related genes. RESULTS: We find that the expression of BACH1 mRNA and protein in prostate cancer tissues is significantly higher than in matched normal prostate tissues (p < .05). In addition, DU145 and LNCaP cells exhibited 4.25-fold and 3.45-fold higher levels of BACH1 compared to HFF cell line. BACH1-siRNA significantly reduced both mRNA and protein expression levels in DU145 cells. More importantly, we show that BACH1 promotes key features of metastasis, as BACH1-siRNA treatment significantly reduced cell invasion and migration by changing the expression levels of a number of metastasis-related genes in vitro. CONCLUSIONS:
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Authors | Neda Shajari, Sadaf Davudian, Tohid Kazemi, Behzad Mansoori, Shima Salehi, Vahid Khaze Shahgoli, Dariush Shanehbandi, Ali Mohammadi, Pascal H G Duijf, Behzad Baradaran |
Journal | Artificial cells, nanomedicine, and biotechnology
(Artif Cells Nanomed Biotechnol)
Vol. 46
Issue 7
Pg. 1495-1504
(11 2018)
ISSN: 2169-141X [Electronic] England |
PMID | 28889753
(Publication Type: Journal Article)
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Chemical References |
- BACH1 protein, human
- Basic-Leucine Zipper Transcription Factors
- RNA, Messenger
- RNA, Small Interfering
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Topics |
- Basic-Leucine Zipper Transcription Factors
(deficiency, genetics, metabolism)
- Cell Movement
(genetics)
- Gene Expression Regulation, Neoplastic
(genetics)
- Gene Silencing
- Humans
- Male
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Prostatic Neoplasms
(pathology)
- RNA, Messenger
(genetics, metabolism)
- RNA, Small Interfering
(genetics)
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