Protective efficacy of a hydroxy fatty acid against gastric Helicobacter infections.

Abstract

BACKGROUND:

We have previously revealed that omega-3 polyunsaturated fatty acids can prevent Helicobacter pylori infection by blocking the futalosine pathway, an alternative route for menaquinone (MK) biosynthesis.

MATERIALS AND METHODS:

1, Different H. pylori strains were grown in liquid media supplemented with linoleic acid, an omega-6 fatty acid, or its 10-hydroxy derivative, 10-hydroxy-cis-12-octadecenoic acid (HYA), in the presence or absence of MK. The bacterial numbers in the media were estimated by plating; 2, C57BL/6NCrl mice received drinking water supplemented with different fatty acids starting from 1 week before infection with H. pylori or Helicobacter suis until the end of the experiment. The gastric colonization levels of H. pylori or H. suis were determined 2 weeks after infection by plating or quantitative PCR, respectively; 3, Mice were given HYA, starting 1 week before infection with H. suis and continuing until 6 months after infection, for analysis of the gastric conditions.

RESULTS:

1, A low concentration (20 μmol/L) of HYA in culture broth suppressed the growth of H. pylori, and this inhibition was reduced by MK supplementation; 2, HYA treatment protected mice against H. pylori or H. suis infection; 3, HYA treatment suppressed the formation of lymphoid follicles in the gastric mucus layer after H. suis infection.

CONCLUSIONS:

HYA prevents gastric Helicobacter infections by blocking their futalosine pathways. Daily HYA supplementation is effective for the prevention of gastric mucosa-associated lymphoid tissue lymphoma induced by persistent infection with H. suis.

• Authors: Hidenori Matsui, Tetsufumi Takahashi, Somay Y Murayama, Marina Kawaguchi, Koichi Matsuo, Masahiko Nakamura
• Journal: Helicobacter (Helicobacter) Vol. 22 Issue 6 (Dec 2017) ISSN: 1523-5378 [Electronic] England
• PMID: 28834011 (Publication Type: Journal Article)
• Copyright: © 2017 John Wiley & Sons Ltd.

Chemical References

• Fatty Acids, Omega-6
• Stearic Acids
• Vitamin K 2
• 10-hydroxystearic acid

Topics

• Animals
• Bacterial Load
• Colony Count, Microbial
• Disease Models, Animal
• Fatty Acids, Omega-6 (administration & dosage)
• Female
• Helicobacter Infections (drug therapy, prevention & control)
• Helicobacter heilmannii (drug effects)
• Helicobacter pylori (drug effects)
• Mice, Inbred C57BL
• Real-Time Polymerase Chain Reaction
• Stearic Acids (administration & dosage)
• Treatment Outcome
• Vitamin K 2 (administration & dosage)