Expression of Serum Exosomal and Esophageal MicroRNA in Rat Reflux Esophagitis.
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| Abstract |
Gastroesophageal reflux disease (GERD) is a common upper gastrointestinal disease. However, the role of exosomal microRNAs (miRNAs) and esophageal miRNAs in GERD has not been studied. A rat model of acid reflux esophagitis was used to establish a novel diagnosis marker for GERD and examine dynamics of miRNA expression in GERD. Rats were sacrificed 3 (acute phase), 7 (sub-acute phase) and 21 days (chronic phase) after induction of esophagitis. Exosomes were extracted from serum, and the expression patterns of serum miRNAs were analyzed. Four upregulated miRNAs (miR-29a-3p, 128-3p, 223-3p and 3473) were identified by microarray analysis. The expression levels of exosomal miR-29a-3p were significantly higher in the chronic phase of reflux esophagitis compared with controls, and increased expression of miR-29a-3p was specific to chronic reflux esophagitis. Esophageal miR-223-3p expression was higher compared with controls, and gradually decreased from acute to chronic phase in esophagitis. In conclusion, exosomal miR-29a-3p and esophageal miR-223-3p might play roles in GERD.
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| Authors | Risa Uemura, Yoshiki Murakami, Atsushi Hashimoto, Akinari Sawada, Koji Otani, Koichi Taira, Shuhei Hosomi, Yasuaki Nagami, Fumio Tanaka, Noriko Kamata, Hirokazu Yamagami, Tetsuya Tanigawa, Toshio Watanabe, Y-H Taguchi, Yasuhiro Fujiwara |
| Journal | International journal of molecular sciences (Int J Mol Sci) Vol. 18 Issue 8 (Jul 25 2017) ISSN: 1422-0067 [Electronic] Switzerland |
| PMID | 28757556 (Publication Type: Journal Article) |
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