Drug resistance and Mycobacterium tuberculosis strain diversity in TB/HIV co-infected patients in Ho Chi Minh city, Vietnam.

Abstract

BACKGROUND:

Mycobacterium tuberculosis strain diversity and drug resistance among people living with human immunodeficiency virus (HIV) in Vietnam have not been described previously.

METHODS:

We examined M. tuberculosis isolates from TB/HIV co-infected patients in Ho Chi Minh City, Vietnam. Drug susceptibility testing (DST), spoligotyping and 24-locus Mycobacterial Interspersed Repetitive Unit (MIRU-24 typing) were performed, and the rpoB, katG, inhA and inhA promoter, rpsL, rrs and embB genes were sequenced in all drug resistant isolates identified.

RESULTS:

In total, 84/200 (42.0%) strains demonstrated "any drug resistance"; 17 (8.5%) were multi-drug resistant (MDR). Streptomycin resistance was present in 80 (40.0%) isolates; 95.2% (80/84) with "any drug resistance" and 100% with MDR. No rifampicin monoresistance was detected. Of the rifampicin resistant strains 16/18 (88.9%) had mutations in the 81-bp Rifampicin Resistance Defining Region (RRDR) of the rpoB gene. Isoniazid resistance was mostly associated with Ser315Thr mutations in the katG gene (15/17; 88.2%). Beijing (49.0%) and East African Indian (EAI) lineage strains (35.0%; 56/70 EAI-5) were most common.

CONCLUSION:

TB/HIV co-infection in Vietnam was associated with high rates of TB drug resistance, although we were unable to differentiate new from retreatment cases.

• Authors: Trinh Quynh Mai, Nguyen Thi Van Anh, Nguyen Tran Hien, Nguyen Huu Lan, Do Chau Giang, Pham Thi Thu Hang, Nguyen Thi Ngoc Lan, Ben J Marais, Vitali Sintchenko
• Journal: Journal of global antimicrobial resistance (J Glob Antimicrob Resist) Vol. 10 Pg. 154-160 (09 2017) ISSN: 2213-7173 [Electronic] Netherlands
• PMID: 28743648 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Crown Copyright © 2017. Published by Elsevier Ltd. All rights reserved.

Chemical References

• Antitubercular Agents
• Bacterial Proteins
• rpoB protein, Mycobacterium tuberculosis
• Oxidoreductases
• Catalase
• katG protein, Mycobacterium tuberculosis
• InhA protein, Mycobacterium
• EmbB protein, Mycobacterium tuberculosis
• Pentosyltransferases
• DNA-Directed RNA Polymerases

Topics

• Adolescent
• Adult
• Antitubercular Agents (pharmacology)
• Bacterial Proteins (genetics)
• Catalase (genetics)
• Coinfection
• DNA-Directed RNA Polymerases (genetics)
• Drug Resistance, Multiple, Bacterial (genetics)
• Female
• Genes, Bacterial (genetics)
• HIV Infections (complications, epidemiology)
• Humans
• Male
• Microbial Sensitivity Tests
• Middle Aged
• Mutation
• Mycobacterium tuberculosis (drug effects, genetics, isolation & purification, pathogenicity)
• Oxidoreductases (genetics)
• Pentosyltransferases (genetics)
• Phylogeny
• Tuberculosis, Multidrug-Resistant (complications, epidemiology, microbiology)
• Vietnam (epidemiology)
• Young Adult