Dietary PUFAs reduce
atherosclerosis and macrophage
inflammation, but how
nucleotide-binding oligomerization domain
leucine-rich repeat-containing receptor
protein (NLRP3)
inflammasome activation and autophagy influence PUFA-mediated atheroprotection is poorly understood. We fed Ldlr-/- mice diets containing 10% (calories)
palm oil (PO) and 0.2%
cholesterol, supplemented with an additional 10% of calories as PO,
fish oil (FO), echium oil (EO, containing 18:4 n-3), or
borage oil (BO, containing 18:3 n-6).
Inflammasome activation, autophagic flux, and mitochondrial function were measured in peritoneal macrophages, blood monocytes, or liver from diet-fed mice. Compared with PO, dietary PUFAs (FO, EO, or BO) markedly inhibited
inflammasome activation, shown by 1) less macrophage IL-1β secretion and
caspase-1 cleavage in response to NLRP3
inflammasome activators, 2) less IL-1β secretion and
caspase-1 cleavage from liver or hepatocytes in response to
lipopolysaccharide (LPS), and 3) attenuated
caspase-1 activity in blood monocytes. Furthermore, PUFA-enriched diets increased LC3-II expression in macrophage, aorta, and liver samples and reduced numbers of dysfunctional mitochondria in macrophages in response to LPS and
palmitate, suggesting enhanced autophagic activation. Dietary PUFAs did not attenuate NLRP3
inflammasome activation in atg5-deficient macrophages, indicating that autophagic activation is critical for the PUFA-mediated
inflammasome inactivation. In conclusion, dietary PUFAs reduce
atherosclerosis, in part, by activation of macrophage autophagy and attenuation of NLRP3
inflammasome activation.