Methoxyamine Enhances 5-Fluorouracil-Induced Radiosensitization in Colon Cancer Cell Line HT29.

Abstract

OBJECTIVE:

This study intended to observe the effects of methoxyamine (Mx) on cytotoxic effects and DNA damage caused by 5-Fluorouracil (5-FU) in combination with gamma radiation in a human colon cancer cell line, HT29.

MATERIALS AND METHODS:

In this experimental study, HT29 cells were cultured as a monolayer and treated with different concentrations of 5-FU along with 1 mM Mx for 24 hours. Next, the cells were irradiated with 2 Gy gamma radiation. After the treatments, we assessed for DNA damage, cytotoxicity, and viability by alkaline comet, clonogenic survival, and trypan blue dye exclusion assays.

RESULTS:

Cytotoxicity and DNA damage increased with increasing 5-FU concentration. The 1 mM Mx concentration had no significant effect on cytotoxicity and DNA damage from 5-FU; however, it increased the cytotoxic and genotoxic effects of different concentrations of 5-FU when used in combination with 2 Gy gamma radiation.

CONCLUSION:

Mx combined with 5-FU enhanced the radiosensitivity of colon cancer cells.

• Authors: Samideh Khoei, Roghayeh Poorabdollahi, Ahmad Mostaar, Fariborz Faeghi
• Journal: Cell journal (Cell J) 2017 Jul-Sep Vol. 19 Issue 2 Pg. 283-291 ISSN: 2228-5806 [Print] Iran
• PMID: 28670521 (Publication Type: Journal Article)