Abstract | BACKGROUND: METHODS: RESULTS: LPS exposure increased CD3-positive and myeloperoxidase-positive cells. Combined UP-LPS exposure increased pulmonary inflammation compared with 2d LPS or UP groups. The UP+2d LPS group had an increased adventitial fibrosis score when compared with UP-treated animals. A reduced wall-to-lumen ratio was found in the 7d LPS animals when compared to the 2d LPS-treated animals. Exposure to UP+2d LPS reduced VEGF and VEGFR-2 levels compared with 2d LPS-treated animals. Angiopoietin-1 (Ang1) and tunica interna endothelial cell kinase 2 (Tie-2) levels were decreased after UP+7d LPS as well as after 7d LPS, but not with UP alone. CONCLUSION: Chronic UP and subsequent LPS exposure increased pulmonary inflammation and decreased expression of angiogenic growth factors and receptors when compared to single hit-exposed animals.
|
Authors | Monique G M Willems, Matthew W Kemp, Laura A Fast, Nick M M Wagemaker, Leon E W Janssen, John P Newnham, Matt S Payne, Owen B Spiller, Suhas G Kallapur, Alan H Jobe, Tammo Delhaas, Boris W Kramer, Tim G A M Wolfs |
Journal | PloS one
(PLoS One)
Vol. 12
Issue 6
Pg. e0180114
( 2017)
ISSN: 1932-6203 [Electronic] United States |
PMID | 28666032
(Publication Type: Journal Article)
|
Chemical References |
|
Topics |
- Animals
- Blood Vessels
(drug effects, microbiology)
- Female
- Lipopolysaccharides
(pharmacology)
- Lung
(blood supply)
- Maternal Exposure
- Models, Animal
- Pregnancy
- Sheep
(embryology, growth & development)
- Ureaplasma
(physiology)
|