Effect of prepartum energy balance on neutrophil function following pegbovigrastim treatment in periparturient cows.

Abstract

Treatment with granulocyte colony-stimulating factor (G-CSF) increases polymorphonuclear cell (neutrophil) count and enhances neutrophil function in the periparturient cow. Prepartum undernutrition was hypothesized to reduce the effect of a commercially available recombinant bovine G-CSF product (pegbovigrastim) on neutrophil count and function. Hence this study was undertaken to test the effect of undernutrition for approximately 1 mo before calving on the innate immune response to pegbovigrastim. Cows (n = 99) on pasture were blocked by expected calving date and body condition score and randomly assigned in a 2 × 2 factorial design. The first factor was that cows were fed to exceed energy requirements prepartum (full feeding) or restricted to approximately 85% of prepartum energy requirements (restricted feeding). The second factor was that at approximately 7 d before expected calving date, half the cows in each feed group were injected with pegbovigrastim and the remaining half were injected with saline. Treatments were repeated on the day of calving. Blood samples were collected pre- and postcalving for complete blood count, biochemistry, and in vitro assessment of neutrophil function including phagocytosis, myeloperoxidase release, and oxidative burst. Prepartum energy restriction resulted in lower body weight, a higher proportion of cows with elevated concentrations (i.e., >0.4 mmol/L) of fatty acids, and higher average β-hydroxybutyrate concentrations before calving relative to fully fed cows. Treatment with pegbovigrastim increased the total white cell, neutrophil, lymphocyte, and monocyte counts. Pegbovigrastim treatment resulted in increased release of myeloperoxidase by neutrophils. Prepartum feeding group did not have an effect, and no feeding group × treatment interaction was observed for any of the white cell counts or functional tests. We concluded that pegbovigrastim treatment results in significant increases in neutrophil count and enhances neutrophil function as indicated by increased myeloperoxidase release. The response to pegbovigrastim was not affected by restricted prepartum energy intake.

• Authors: S McDougall, S J LeBlanc, A Heiser
• Journal: Journal of dairy science (J Dairy Sci) Vol. 100 Issue 9 Pg. 7478-7492 (Sep 2017) ISSN: 1525-3198 [Electronic] United States
• PMID: 28647326 (Publication Type: Journal Article)
• Copyright: The Authors. Published by the Federation of Animal Science Societies and Elsevier Inc. on behalf of the American Dairy Science Association®. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/3.0/).

Chemical References

• Fatty Acids, Nonesterified
• Recombinant Proteins
• Granulocyte Colony-Stimulating Factor
• Polyethylene Glycols
• pegylated granulocyte colony-stimulating factor

Topics

• Animals
• Cattle
• Diet
• Energy Intake
• Energy Metabolism
• Fatty Acids, Nonesterified
• Female
• Granulocyte Colony-Stimulating Factor (pharmacology)
• Immunity, Innate (drug effects)
• Lactation
• Leukocyte Count (veterinary)
• Malnutrition (immunology, veterinary)
• Neutrophils (drug effects, physiology)
• Parturition
• Polyethylene Glycols (pharmacology)
• Pregnancy
• Random Allocation
• Recombinant Proteins (pharmacology)