Abstract | BACKGROUND: METHODS: Twenty-four rats were divided into three groups: sham operation group (group S), ischemia/reperfusion group (group IR), and lipid emulsion treatment group (group LE). limb ischemia/reperfusion was induced through occlusion of the infrarenal abdominal aorta for 3 h. The microvascular clamp was removed carefully and reperfusion was provided for 3 h. RESULTS: The mean arterial pressure in group LE was higher than group IR during the reperfusion period (P = 0.024). The heart rate of both group LE and IR are significantly higher than group S during the ischemia period(P < 0.001, P < 0.001, respectively). The arterial oxygen pressure of group LE was significantly higher than group IR (P = 0.003), the arterial carbon dioxide pressure of group LE were lower than that of group IR (P = 0.005). The concentration of plasma interleukin-6, tumor necrosis factor-α and malondialdehyde in group LE were significantly lower than group IR (P < 0.001, P = 0.009 and 0.029, respectively). The plasma superoxide dismutase activity in group LE was significantly higher than group IR (P = 0.029). The myeloperoxidase activity in lung tissues of group LE was significantly less than group IR (P = 0.046). Both muscle and lung in group IR were damaged seriously, whereas lipid emulsion ( Intralipid) effectively reversed the damage. In summary, Intralipid administration resulted in several beneficial effects as compared to group IR, such as the pulmonary gas exchange and inflammatory. CONCLUSIONS: The ischemic/ reperfusion injury of limb muscles with resultant inflammatory damage to lung tissue can be mitigated by administration of a lipid emulsion ( Intralipid, 20%, 5 ml/kg). The mechanisms attenuating such a physiological may be attributed to reduction of the degree of limb injury through a decrease in the release of local inflammatory mediators, a reduction of lipid peroxidation, and a blunting of the subsequent remote inflammatory response.
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Authors | Fangfang Xia, Yun Xia, Sisi Chen, Lulu Chen, Weijuan Zhu, Yuanqing Chen, Thomas J Papadimos, Xuzhong Xu, Le Liu |
Journal | BMC anesthesiology
(BMC Anesthesiol)
Vol. 17
Issue 1
Pg. 83
(06 19 2017)
ISSN: 1471-2253 [Electronic] England |
PMID | 28629353
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Cytokines
- Emulsions
- Fat Emulsions, Intravenous
- Phospholipids
- soybean oil, phospholipid emulsion
- Soybean Oil
- Peroxidase
- Superoxide Dismutase
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Topics |
- Acute Lung Injury
(etiology, metabolism, therapy)
- Animals
- Cytokines
(blood)
- Emulsions
(pharmacology)
- Fat Emulsions, Intravenous
(pharmacology)
- Lung
(metabolism)
- Oxidative Stress
- Peroxidase
(metabolism)
- Phospholipids
(pharmacology)
- Rats, Sprague-Dawley
- Reperfusion Injury
(complications)
- Soybean Oil
(pharmacology)
- Superoxide Dismutase
(blood)
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