Hemorrhagic shock following major
trauma results in mortality, but the function of vascular adhesion protein‑1 (VAP‑1), implicated in
intracranial hemorrhage, remains unknown. This study aimed to determine whether expression of the AOC3 gene and its encoded
protein, VAP‑1, is altered by
hypoxia. Rat hepatic sinusoidal endothelial cells (RHSECs) and rat intestinal microvascular endothelial cells (RIMECs) were transduced with a viral vector carrying AOC3, and AOC3
mRNA expression levels were measured by reverse transcription‑quantitative polymerase chain reaction. VAP‑1
protein expression levels were measured by western blot analysis and compared between normoxic and hypoxic conditions. Following this, AOC3
mRNA and VAP‑1
protein expression levels in hepatic and intestinal tissues were assessed in a rat model of
hemorrhagic shock with or without fluid
resuscitation; and serum semicarbazide‑sensitive
amine oxidase (SSAO) activity was measured by fluorometric assays. The effects of 2‑bromoethylamine (2‑BEA) on AOC3/VAP‑1 levels and 24 h survival were investigated. AOC3
mRNA and VAP‑1
protein levels were increased in RHSECs and RIMECs by
hypoxia, and in hepatic and intestinal tissues from rats following
hemorrhagic shock.
Hypoxia increased serum SSAO activity in these animals. 2‑BEA reduced AOC3
mRNA and VAP‑1
protein levels in hepatic and intestinal tissues from rats following
hemorrhagic shock, and appeared to improve survival in animals not receiving
resuscitation following
hemorrhagic shock. In conclusion,
hemorrhagic shock upregulates AOC3/VAP‑1 expressions, and this potentially occurs via
hypoxia. Therefore, inhibition of VAP‑1 may be beneficial in the setting of
hemorrhagic shock. Further studies are required to confirm these findings and to establish whether VAP‑1 may be a valid target for the development of novel
therapies for
hemorrhagic shock.