[Expressions and Significance of ALDH1, HIF-1α and VEGF in Laryngeal Squamous Cell Carcinoma].

Abstract

OBJECTIVES:

To investigate the expressions of aldehyde dehydrogenase 1(ALDH1), hypoxia-inducible factor 1α(HIF-1α), vascular endothelial growth factor (VEGF) in laryngeal squamous cell carcinoma(LSCC) and their relationships with clinical significance.

METHODS:

In situ hybridization (ISH) method and immuohistochemical staining were used to detect the mRNA and protein expressions of ALDH1, HIF-1α and VEGF in 120 LSCC specimens and 60 cases of normal laryngeal tissue. Their relationships with clinical pathological parameters were analyzed.

RESULTS:

The positive rates of ALDH1, HIF-1α and VEGF mRNA in LSCC were 57.5% (69/120) , 73.3% (88/120) , 56.7% (68/120) , while the positive rates of proteins were 55.8% (67/120) , 70.8% (85/120) and 55.0% (66/120) , respectively. which were significantly higher than those in controls (P<0.05) . The expression of ALDH1 and VEGF proteins were significantly correlated with TNM stage and lymph node metastasis (P<0.05) . The expression of HIF-1α protein was significantly correlated with TNM stage, the degree of differentiation and lymph node metastasis (P<0.05). The positive expression of ALDH1 was positively associated with those of HIF-1α and VEGF (P<0.05). The lymph node metastasis rate increased with the positive factors. Kaplan-Meier survival analysis showed that the patients with positive expression of ALDH1, HIF-1α and VEGF had lower 5-year overall survival rate than those with negative expression (P<0.05) .

CONCLUSIONS:

The expressions of ALDH1, HIF-1α and VEGF are higher in LSCC, which may be used to determine the malignancy and prognosis of LSCC.

• Authors: Yan Zhao, Xin Jin, Nan Li, Yan-Zi Qin, Jun Qian
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 48 Issue 3 Pg. 399-404 (May 2017) ISSN: 1672-173X [Print] China
• PMID: 28616914 (Publication Type: Journal Article)

Chemical References

• HIF1A protein, human
• Hypoxia-Inducible Factor 1, alpha Subunit
• Isoenzymes
• VEGFA protein, human
• Vascular Endothelial Growth Factor A
• Aldehyde Dehydrogenase 1 Family
• ALDH1A1 protein, human
• Retinal Dehydrogenase

Topics

• Aldehyde Dehydrogenase 1 Family
• Carcinoma, Squamous Cell (metabolism, pathology)
• Humans
• Hypoxia-Inducible Factor 1, alpha Subunit (metabolism)
• Isoenzymes (metabolism)
• Laryngeal Neoplasms (metabolism, pathology)
• Retinal Dehydrogenase (metabolism)
• Vascular Endothelial Growth Factor A (metabolism)