Abstract |
BackgroundCellular oxidative stress, inflammatory responses, and immunogenic events are involved in pathogenesis of idiopathic nephrotic syndrome (INS); however, the exact mechanism remains unknown. We examined NADPH oxidase (NOX) activity and platelet-derived growth factor (PDGF)-induced DNA synthesis in peripheral blood lymphocytes (PBL) of patients with INS.MethodsPBL from 15 patients with INS and 15 age- and gender-matched controls were isolated, and enzyme activities of NOX, catalase, and superoxide dismutase (SOD) were measured along with the assay of malondialdehyde levels and bromo- deoxyuridine incorporation. Protein expression of NOX-1 was measured using western blot analysis.ResultsPatients with INS had significantly (P<0.01) higher NOX activity and increased protein expression of NOX-1 in PBL as compared with controls. Catalase and SOD activities were markedly lower with lipid peroxide levels significantly (P<0.01) increased in patients with INS. Ex vivo DNA synthesis in PDGF-stimulated PBL was significantly (P<0.01) reduced in patients with INS; however, diphenyliodonium, an inhibitor of NOX, markedly corrected impairment in growth factor-induced BrdU incorporation.ConclusionsThese results show that NOX activation might have a role in regulation of lymphocytic activity in patients with INS through the impairment of PDGF mitogenic function and might contribute toward pathogenesis of nephrotic syndrome.
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Authors | Amal Al-Eisa, Gursev S Dhaunsi |
Journal | Pediatric research
(Pediatr Res)
Vol. 82
Issue 4
Pg. 629-633
(Oct 2017)
ISSN: 1530-0447 [Electronic] United States |
PMID | 28613279
(Publication Type: Journal Article)
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Chemical References |
- Lipid Peroxides
- Platelet-Derived Growth Factor
- Malondialdehyde
- Catalase
- Superoxide Dismutase
- NADPH Oxidase 1
- NOX1 protein, human
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Topics |
- Case-Control Studies
- Catalase
(blood)
- Cells, Cultured
- Child
- Child, Preschool
- DNA Replication
(drug effects)
- Enzyme Activation
- Female
- Humans
- Lipid Peroxidation
(drug effects)
- Lipid Peroxides
(blood)
- Lymphocytes
(drug effects, enzymology)
- Male
- Malondialdehyde
(blood)
- NADPH Oxidase 1
(blood)
- Nephrotic Syndrome
(blood, diagnosis, enzymology)
- Oxidative Stress
(drug effects)
- Platelet-Derived Growth Factor
(pharmacology)
- Superoxide Dismutase
(blood)
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