Abstract | PURPOSE: To investigate the protective effect of LPS preconditioning against EIU in rats. METHODS: EIU in Wistar rats was developed by subcutaneous injection of LPS (200 μg). Lower dose of LPS (0.1 mg/kg, intraperitoneally) or its carrier was injected daily for five days before EIU induction. Twenty-four hours after EIU, eyes were examined and then enucleated. The degree of inflammatory reaction was determined by routine histological examinations. Real-time RT-PCR and Western blot were used to determine the activation of NF-kB and expression of IRAK-1, IRAK-4, and IRAK-M Results: Repeated pre-administration of LPS induced a significant reduction in ocular inflammation and the expression of NF-κb p65 in neurons. The expression of IRAK-1 and IRAK-4 was suppressed in endotoxin tolerance group, whereas IRAK-M was increased. CONCLUSIONS: Endotoxin tolerance has a protective effect against EIU, and upregulation of IRAK-M through TLR-signaling pathway is one of the most likely candidates to be involved in the observed phenomenon.
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Authors | Shuo Yu, Xinli Liu, Nan Zhang, Shuo Yang, Cui Mao, Shilan Feng, Hong Lu |
Journal | Ocular immunology and inflammation
(Ocul Immunol Inflamm)
Vol. 26
Issue 6
Pg. 943-950
( 2018)
ISSN: 1744-5078 [Electronic] England |
PMID | 28609207
(Publication Type: Journal Article)
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Chemical References |
- Endotoxins
- Lipopolysaccharides
- RNA
- IRAK1 protein, rat
- Interleukin-1 Receptor-Associated Kinases
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Topics |
- Animals
- Aqueous Humor
(enzymology)
- Blotting, Western
- Disease Models, Animal
- Dose-Response Relationship, Drug
- Endotoxins
(toxicity)
- Gene Expression Regulation
- Interleukin-1 Receptor-Associated Kinases
(biosynthesis, genetics)
- Lipopolysaccharides
(administration & dosage)
- Male
- RNA
(genetics)
- Rats
- Rats, Wistar
- Real-Time Polymerase Chain Reaction
- Uveitis
(chemically induced, genetics, prevention & control)
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