Abstract |
The present study tests a hypothesis that cardioprotective effects mediated by autologous adipose-derived stem cells (ADSC) in rats afflicted with insulin-dependent diabetes mellitus ( IDDM) may be synergistically enhanced by oral treatment with green tea epigallocatechin gallate (EGCG). Wistar rats were divided into sham, DM, DM+ADSC (autologous transplanted 1 × 106 cells per rat), and DM+ADSC+E (E, green tea oral administration EGCG). Heart tissues were isolated from all rats, and investigations were performed after 2-mo treatment. In the sham, DM, and DM+ADSC groups, we found that DM induced cardiac dysfunction ( sham and DM) and autologous ADSC transplantation could partially recover cardiac functions (DM and DM+ADSC) in DM rats. Compared with DM+ADSC, significant improvement in cardiac functions can be observed in DM+ADSC+E in echocardiographic data, histological observations, and even cellular protein expression. Oral green tea EGCG administration and autologous ADSC transplantation show synergistically beneficial effects on diabetic cardiac myopathy in DM rats.NEW & NOTEWORTHY Cardiomyopathy can be induced in rats with diabetes mellitus (DM). Heart function can be restored in DM rats with adipose-derived stem cell treatment. Oral epigallocatechin gallate (EGCG) administration synergistically enhances cardiac function in DM rats with stem cell treatment. The EGCG and stem cell treatment cross-effect occurs via survival protein expression.
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Authors | Tung-Sheng Chen, Show-Yih Liou, Chia-Hua Kuo, Lung-Fa Pan, Yu-Lan Yeh, Jeffery Liou, V Vijaya Padma, Chun-Hsu Yao, Wei-Wen Kuo, Chih-Yang Huang |
Journal | Journal of applied physiology (Bethesda, Md. : 1985)
(J Appl Physiol (1985))
Vol. 123
Issue 5
Pg. 1081-1091
(Nov 01 2017)
ISSN: 1522-1601 [Electronic] United States |
PMID | 28546469
(Publication Type: Journal Article)
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Copyright | Copyright © 2017 the American Physiological Society. |
Chemical References |
- Biomarkers
- Blood Glucose
- Catechin
- epigallocatechin gallate
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Topics |
- Adipose Tissue
(cytology, metabolism, transplantation)
- Administration, Oral
- Animals
- Apoptosis
(drug effects)
- Biomarkers
(metabolism)
- Blood Glucose
(metabolism)
- Catechin
(administration & dosage, analogs & derivatives)
- Cell Survival
(drug effects)
- Cells, Cultured
- Combined Modality Therapy
- Diabetes Mellitus, Experimental
(blood, complications, therapy)
- Diabetic Cardiomyopathies
(diagnostic imaging, etiology, physiopathology, prevention & control)
- Echocardiography
- Male
- Myocytes, Cardiac
(drug effects, metabolism, pathology)
- Rats, Wistar
- Recovery of Function
- Signal Transduction
(drug effects)
- Stem Cell Transplantation
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