Abstract |
Hpn is a small histidine-rich cytoplasmic protein from Helicobacter pylori and has been recognized as a high-risk factor for several cancers including gastric cancer, colorectal cancer, and MALT lymphoma. However, the relationship between Hpn and cancers remains elusive. In this study, we discovered that Hpn protein effectively suppressed cell growth and induced apoptosis in hepatocellular carcinoma (HCC). A two-dimensional gel electrophoresis and mass spectrometry-based comparative proteomics was performed to find the molecular targets of Hpn in HCC cells. It was identified that twelve proteins were differentially expressed, with USP5 being one of the most significantly downregulated protein. The P14ARF -P53 signaling was activated by USP5 knockdown in HCC cells. Furthermore, USP5 overexpression significantly rescued the suppressive effect of Hpn on the viability of HCC cells. In conclusion, our study suggests that Hpn plays apoptosis-inducing roles through suppressing USP5 expression and activating the P14ARF -P53 signaling. Therefore, Hpn may be a potential candidate for developing novel anti-HCC drugs.
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Authors | Yi Liu, Wei-Mao Wang, Li-Yi Zou, Li Li, Lu Feng, Ming-Zhu Pan, Min-Yi Lv, Ying Cao, Hua Wang, Hsiang-Fu Kung, Jian-Xin Pang, Wei-Ming Fu, Jin-Fang Zhang |
Journal | Proteomics
(Proteomics)
Vol. 17
Issue 12
(Jun 2017)
ISSN: 1615-9861 [Electronic] Germany |
PMID | 28523650
(Publication Type: Journal Article)
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Copyright | © 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. |
Chemical References |
- CDK2AP2 protein, human
- Oncogene Proteins
- Proteins
- TP53 protein, human
- Tumor Suppressor Protein p53
- histidine-rich proteins
- Endopeptidases
- ubiquitin isopeptidase
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Topics |
- Apoptosis
- Carcinoma, Hepatocellular
(metabolism, pathology)
- Cell Line, Tumor
- Cell Survival
- Endopeptidases
(metabolism)
- Genes, Tumor Suppressor
- Humans
- Liver Neoplasms
(metabolism, pathology)
- Oncogene Proteins
(metabolism)
- Proteins
(metabolism)
- Proteomics
(methods)
- Signal Transduction
- Tumor Suppressor Protein p53
(metabolism)
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