Abstract |
Epstein-Barr virus (EBV) replication contributes to multiple human diseases, including infectious mononucleosis, nasopharyngeal carcinoma, B cell lymphomas, and oral hairy leukoplakia. We performed systematic quantitative analyses of temporal changes in host and EBV proteins during lytic replication to gain insights into virus-host interactions, using conditional Burkitt lymphoma models of type I and II EBV infection. We quantified profiles of >8,000 cellular and 69 EBV proteins, including >500 plasma membrane proteins, providing temporal views of the lytic B cell proteome and EBV virome. Our approach revealed EBV-induced remodeling of cell cycle, innate and adaptive immune pathways, including upregulation of the complement cascade and proteasomal degradation of the B cell receptor complex, conserved between EBV types I and II. Cross-comparison with proteomic analyses of human cytomegalovirus infection and of a Kaposi-sarcoma-associated herpesvirus immunoevasin identified host factors targeted by multiple herpesviruses. Our results provide an important resource for studies of EBV replication.
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Authors | Ina Ersing, Luis Nobre, Liang Wei Wang, Lior Soday, Yijie Ma, Joao A Paulo, Yohei Narita, Camille W Ashbaugh, Chang Jiang, Nicholas E Grayson, Elliott Kieff, Steven P Gygi, Michael P Weekes, Benjamin E Gewurz |
Journal | Cell reports
(Cell Rep)
Vol. 19
Issue 7
Pg. 1479-1493
(05 16 2017)
ISSN: 2211-1247 [Electronic] United States |
PMID | 28514666
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2017 The Author(s). Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Receptors, Antigen, B-Cell
- Transcription Factors
- Complement System Proteins
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Topics |
- B-Lymphocytes
(metabolism, virology)
- Cell Cycle
- Cell Membrane
(metabolism)
- Complement System Proteins
(metabolism)
- Down-Regulation
- Herpesvirus 4, Human
(physiology)
- Humans
- Proteolysis
- Proteomics
(methods)
- Receptors, Antigen, B-Cell
(metabolism)
- Time Factors
- Transcription Factors
(metabolism)
- Up-Regulation
- Virus Replication
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