Study of rubella candidate vaccine based on a structurally modified plant virus.

Abstract

A novel rubella candidate vaccine based on a structurally modified plant virus - spherical particles (SPs) - was developed. SPs generated by the thermal remodelling of the tobacco mosaic virus are promising platforms for the development of vaccines. SPs combine unique properties: biosafety, stability, high immunogenicity and the effective adsorption of antigens. We assembled in vitro and characterised complexes (candidate vaccine) based on SPs and the rubella virus recombinant antigen. The candidate vaccine induced a strong humoral immune response against rubella. The IgG isotypes ratio indicated the predominance of IgG1 which plays a key role in immunity to natural rubella infection. The immune response was generally directed against the rubella antigen within the complexes. We suggest that SPs can act as a platform (depot) for the rubella antigen, enhancing specific immune response. Our results demonstrate that SPs-antigen complexes can be an effective and safe candidate vaccine against rubella.

• Authors: Ekaterina A Trifonova, Vladimir A Zenin, Nikolai A Nikitin, Maria S Yurkova, Ekaterina M Ryabchevskaya, Egor V Putlyaev, Ekaterina K Donchenko, Olga A Kondakova, Alexey N Fedorov, Joseph G Atabekov, Olga V Karpova
• Journal: Antiviral research (Antiviral Res) Vol. 144 Pg. 27-33 (08 2017) ISSN: 1872-9096 [Electronic] Netherlands
• PMID: 28511994 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2017 Elsevier B.V. All rights reserved.

Chemical References

• Antibodies, Viral
• Drug Carriers
• Immunoglobulin G
• Rubella Vaccine
• Vaccines, Synthetic

Topics

• Animals
• Antibodies, Viral (blood)
• Drug Carriers
• Immunoglobulin G (blood)
• Mice, Inbred BALB C
• Rubella Vaccine (administration & dosage, genetics, immunology)
• Rubella virus (genetics, immunology)
• Tobacco Mosaic Virus (genetics)
• Vaccines, Synthetic (administration & dosage, genetics, immunology)