Study of Hydroquinone Mediated Cytotoxicity and Hypopigmentation Effects from UVB-Irradiated Arbutin and DeoxyArbutin.

Abstract

Arbutin (Arb) and deoxyArbutin (dA) are both effective hypopigmentation agents. However, they are glucoside derivatives of hydroquinone (HQ), which may be decayed into HQ under higher energy environments. Therefore, safety and toxicity are very important issues when considering the usage of these compounds. However, no study has verified the properties of Ultra-Violet B (UVB)-irradiated Arb and dA. In this work, we investigated the cytotoxicity and hypopigmentation effects of UVB-irradiated Arb and dA in Detroit 551 human fibroblast cells and B16-F10 mouse melanoma cells. The results showed that UVB-irradiated Arb and dA have strong cytotoxicity for the fibroblast cells, especially for dA, the caspase-3 is also activated by the treatment of UVB-irradiated dA in Detroit 551 cells. The results correlated with the produced HQ. In addition, UVB-irradiated Arb and dA suppressed the production of melanin in melanoma cells; this is due to the release of HQ that compensates for the UVB triggered Arb and dA decomposition.

• Authors: Nai-Fang Chang, Yi-Shyan Chen, Ying-Ju Lin, Ting-Hsuan Tai, An-Ni Chen, Chen-Hsuan Huang, Chih-Chien Lin
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 18 Issue 5 (May 03 2017) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 28467382 (Publication Type: Journal Article)

Chemical References

• Glucosides
• Hydroquinones
• Melanins
• Arbutin
• CASP3 protein, human
• Caspase 3
• deoxyarbutin
• hydroquinone

Topics

• Animals
• Arbutin (analogs & derivatives, radiation effects, toxicity)
• Caspase 3 (drug effects)
• Cell Survival (drug effects)
• Cells, Cultured
• Fibroblasts (drug effects)
• Glucosides
• Humans
• Hydroquinones (radiation effects, toxicity)
• Hypopigmentation (chemically induced)
• Melanins (antagonists & inhibitors)
• Melanocytes (drug effects)
• Melanoma, Experimental
• Mice
• Ultraviolet Rays