Abstract |
Fragile X-associated tremor/ataxia syndrome ( FXTAS) is a neurological disorder that affects premutation carriers with 55-200 CGG-expansion repeats (preCGG) in FMR1, presenting with early alterations in neuronal network formation and function that precede neurodegeneration. Whether intranuclear inclusions containing DNA damage response ( DDR) proteins are causally linked to abnormal synaptic function, neuronal growth and survival are unknown. In a mouse that harbors a premutation CGG expansion (preCGG), cortical and hippocampal FMRP expression is moderately reduced from birth through adulthood, with greater FMRP reductions in the soma than in the neurite, despite several-fold elevation of Fmr1 mRNA levels. Resting cytoplasmic calcium concentration ([Ca2+]i) in cultured preCGG hippocampal neurons is chronically elevated, 3-fold compared to Wt; elevated ROS and abnormal glutamatergic responses are detected at 14 DIV. Elevated µ- calpain activity and a higher p25/p35 ratio in the cortex of preCGG young adult mice indicate abnormal Cdk5 regulation. In support, the Cdk5 substrate, ATM, is upregulated by 1.5- to 2-fold at P0 and 6 months in preCGG brain, as is p-Ser1981-ATM. Bax:Bcl-2 is 30% higher in preCGG brain, indicating a greater vulnerability to apoptotic activation. Elevated [Ca2+]i, ROS, and DDR signals are normalized with dantrolene. Chronic [Ca2+]i dysregulation amplifies Cdk5-ATM signaling, possibly linking impaired glutamatergic signaling and DDR to neurodegeneration in preCGG brain.
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Authors | Gaëlle Robin, José R López, Glenda M Espinal, Susan Hulsizer, Paul J Hagerman, Isaac N Pessah |
Journal | Human molecular genetics
(Hum Mol Genet)
Vol. 26
Issue 14
Pg. 2649-2666
(07 15 2017)
ISSN: 1460-2083 [Electronic] England |
PMID | 28444183
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)
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Copyright | © The Author 2017. Published by Oxford University Press. All rights reserved. For Permissions, please email: [email protected]. |
Chemical References |
- FMR1 protein, human
- Fmr1 protein, mouse
- RNA, Messenger
- Fragile X Mental Retardation Protein
- ATM protein, human
- Ataxia Telangiectasia Mutated Proteins
- Atm protein, mouse
- Cyclin-Dependent Kinase 5
- CDK5 protein, human
- Cdk5 protein, mouse
- Calcium
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Topics |
- Animals
- Ataxia
(genetics, metabolism)
- Ataxia Telangiectasia Mutated Proteins
(genetics, metabolism)
- Calcium
(metabolism)
- Cyclin-Dependent Kinase 5
(genetics, metabolism)
- Disease Models, Animal
- Female
- Fragile X Mental Retardation Protein
(genetics, metabolism)
- Fragile X Syndrome
(genetics, metabolism)
- Hippocampus
(metabolism, pathology)
- Humans
- Male
- Mice
- Mice, Inbred C57BL
- Neurons
(metabolism, pathology)
- RNA, Messenger
(metabolism)
- Tremor
(genetics, metabolism)
- Trinucleotide Repeat Expansion
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