Abstract | BACKGROUND:
Anaphylactoid reactions, accounting for more than 77% of all immune-mediated immediate hypersensitivity reactions, have become a serious threat to public health, but their effect mechanism is not clear and diagnostic tests are limited. Comprehensive metabolite analysis may reveal the anaphylactoid effect mechanism systematically and provide reference for future diagnostic purposes. METHODS: Plasma from Brown Norway rats given intravenous injection of saline, compound 48/80 (2.5 mL/kg) or ovalbumin (20 mL/kg) in 20 s for the first time was used to study the effect mechanism of anaphylactoid reactions through metabolomics (UPLC-qTOF-MS/MS). Metabolomics integrated with proteomics data were used to analyze the anaphylactoid pathways by MetaboAnalyst followed by integrated pathway analysis. RESULTS: CONCLUSION: The results may provide a reference to improve diagnostic accuracy and predict and monitor treatment efficacy in anaphylactoid reactions in the clinical setting.
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Authors | Yubin Xu, Na Guo, Deqiang Dou, Xiaoku Ran, Chunyan Liu |
Journal | Asian Pacific journal of allergy and immunology
(Asian Pac J Allergy Immunol)
Vol. 35
Issue 4
Pg. 224-232
(Dec 2017)
ISSN: 0125-877X [Print] Thailand |
PMID | 28364409
(Publication Type: Journal Article)
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Chemical References |
- Allergens
- Arachidonic Acid
- Citric Acid
- gamma-glutamylhistamine
- Histamine
- Ovalbumin
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Topics |
- Allergens
(immunology)
- Anaphylaxis
(metabolism)
- Animals
- Arachidonic Acid
(metabolism)
- Citric Acid
(metabolism)
- Disease Models, Animal
- Histamine
(analogs & derivatives, metabolism)
- Humans
- Hypersensitivity
(metabolism)
- Male
- Metabolomics
(methods)
- Ovalbumin
(immunology)
- Proteomics
- Rats
- Rats, Inbred BN
- Signal Transduction
- Tandem Mass Spectrometry
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