ANGPTL8 (Betatrophin) is Expressed in Visceral Adipose Tissue and Relates to Human Hepatic Steatosis in Two Independent Clinical Collectives.

Abstract

Angiopoietin-like protein 8 (ANGPTL8)/betatrophin expression in visceral adipose tissue and associations with circulating fatty acid profile have not yet been investigated.Forty subjects were included in a cross-sectional study, 57 in a dietary weight reduction intervention. Circulating Angiopoietin-like protein 8/betatrophin was measured in all subjects. Liver and adipose tissue were sampled and plasma fatty acids and tissue Angiopoietin-like protein 8/betatrophin expression were evaluated in the cross-sectional study. In the intervention study oral glucose testing and liver magnetic resonance scanning at baseline and after 6 months were performed. Angiopoietin-like protein 8/betatrophin mRNA was increased in visceral compared to subcutaneous adipose tissue (p<0.001). Circulating ANGPTL8/betatrophin correlated with liver steatosis (r=0.42, p=0.047), triacylglycerols (r=0.34, p=0.046), saturated (r=0.43, p=0.022), monounsaturated (r=0.51, p=0.007), and polyunsaturated fatty acids (r=-0.53, p=0.004). In the intervention study, baseline Angiopoietin-like protein 8/betatrophin correlated with age (r=0.32, p=0.010) and triacylglycerols (r=0.30, p=0.02) and was increased with hepatic steatosis (p=0.033). Weight loss reduced liver fat by 45% and circulating Angiopoietin-like protein 8/betatrophin by 11% (288±17 vs. 258±17 pg/ml; p=0.015). Angiopoietin-like protein 8/betatrophin is related to liver steatosis, while visceral adipose tissue represents an additional site of expression in humans.

• Authors: Christian von Loeffelholz, Andreas F H Pfeiffer, Johan F Lock, Steffi Lieske, Stephanie Döcke, Veronica Murahovschi, Jennifer Kriebel, Tonia de Las Heras Gala, Harald Grallert, Natalia Rudovich, Martin Stockmann, Joachim Spranger, Gerhard Jahreis, Stefan R Bornstein, George Lau, Aimin Xu, Jeanette Schulz-Menger, Louisa Exner, Sven Haufe, Jens Jordan, Stefan Engeli, Andreas L Birkenfeld
• Journal: Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme (Horm Metab Res) Vol. 49 Issue 5 Pg. 343-349 (May 2017) ISSN: 1439-4286 [Electronic] Germany
• PMID: 28351093 (Publication Type: Journal Article, Randomized Controlled Trial)
• Copyright: © Georg Thieme Verlag KG Stuttgart · New York.

Chemical References

• ANGPTL8 protein, human
• Angiopoietin-Like Protein 8
• Angiopoietin-like Proteins
• Peptide Hormones
• RNA, Messenger

Topics

• Angiopoietin-Like Protein 8
• Angiopoietin-like Proteins (genetics, metabolism)
• Cohort Studies
• Diet
• Fatty Liver (genetics)
• Female
• Humans
• Intra-Abdominal Fat (metabolism)
• Male
• Middle Aged
• Non-alcoholic Fatty Liver Disease (genetics)
• Peptide Hormones (genetics, metabolism)
• RNA, Messenger (genetics, metabolism)